2 documents found, page 1 of 1
Development and optimization of a new MALDI-TOF protocol for identification of ...
Oliveira, M. M. E.; Santos, C.; Sampaio, Paula; Romeo, Orazio; Almeida-Paes, Rodrigo; Pais, Célia; Lima, Nelson; Zancope-Oliveira, R. M.Accurate species identification of the Sporothrix schenckii complex is essential, since identification based only on phenotypic characteristics is often inconclusive due to phenotypic variability within the species. We used matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for species identification of 70 environmental and clinical isolates of the Sporothrix complex. A ...
Evaluation of T3B fingerprinting for identification of clinical and environment...
Oliveira, Manoel Marques Evangelista de; Franco-Duarte, Ricardo; Romeo, Orazio; Pais, Célia; Criseo, Giuseppe; Sampaio, PaulaIn this study, PCR fingerprinting using the universal primer T3B was applied to distinguish among clinical and environmental species of the Sporothrix complex, Sporothrix brasiliensis, S. globosa, S. mexicana, S. pallida, S. luriei and S. schenckii sensu stricto. The T3B fingerprinting generated clearly distinct banding patterns, allowing the correct identification of all 43 clinical and environmental isolates ...