29 documents found, page 1 of 3
Improving the reference standard for the diagnosis of canine visceral leishmani...
Teixeira, Ana Izabel Passarella; Silva, Debora Marcolino; Vital, Tamires Emanuele; Nitz, Nadjar; Carvalho, Bruna Caroline Véras deBACKGROUND Studies aimed at validating canine visceral leishmaniasis diagnostic tests present heterogeneous results regarding test accuracy, partly due to divergences in reference standards used and different infection evolution periods in animals. OBJECTIVE This study aimed to evaluate the accuracy of the rapid test-dual path platform (TR-DPP) (Biomanguinhos®), EIE-Leishmaniose-Visceral-Canina-Biomanguinhos (E...
Phylloseptin-1 is leishmanicidal for amastigotes of Leishmania amazonensis insi...
Kückelhaus, Selma Aparecida Souza; Aquino, Daniela Sant’Ana de; Borges, Tatiana Karla dos Santos; Moreira, Daniel Carneiro; Leite, Luciana de MagalhãesLeishmania protozoans are the causal agents of neglected diseases that represent an important public health issue worldwide. The growing occurrence of drug-resistant strains of Leishmania and severe side effects of available treatments represent an important challenge for the leishmaniases treatment. We have previously reported the leishmanicidal activity of phylloseptin-1 (PSN-1), a peptide found in the skin s...
Validation and reliability of the rapid diagnostic test ‘SD Bioeasy Dengue Duo’...
Prado, Paulo Sousa; Almeida Júnior, José Teófilo Duarte; Abreu, Lanna Takada de; Silva, Cristina Gabriel; Souza, Larissa da CostaBACKGROUND The diagnosis of dengue is complex. Until recently, only specialised laboratories were able to confirm dengue infection. However, this has changed with the newly available immunochromatographic rapid tests. Early diagnosis is of great interest, and point-of-care rapid tests have been increasingly used in Brazil. Most of those tests have not undergone validation in the Brazilian population. In this co...
Efficacy and safety of amphotericin B deoxycholate versus N-methylglucamine ant...
Borges, Myrlena Mescouto; Pranchevicius, Maria Cristina da Silva; Noronha, Elza Ferreira; Romero, Gustavo Adolfo Sierra; Carranza Tamayo, César OmarIntroduction: despite their high toxicity, antimonials and amphotericin B deoxycholate are commonly used for treating visceral leishmaniasis (VL). Few studies showing conflictive data about their efficacy and adverse events in pediatric population are available. This study aimed to evaluate efficacy and safety of amphotericin B deoxycholate vs. that of N-methylglucamine antimoniate in treating pediatric VL in B...
G6PD deficiency in Latin America : systematic review on prevalence and variants
Monteiro, Wuelton Marcelo; Val, Fernando Fonseca de Almeida; Siqueira, André Machado; Franca, Gabriel Peixoto; Sampaio, Vanderson de SouzaPlasmodium vivax radical cure requires the use of primaquine (PQ), a drug that induces haemolysis in glucose-6-phosphate dehydrogenase deficient (G6PDd) individuals, which further hampers malaria control efforts. The aim of this work was to study the G6PDd prevalence and variants in Latin America (LA) and the Caribbean region. A systematic search of the published literature was undertaken in August 2013. Biblio...
Leishmania (Viannia) naiffi : rare enough to be neglected?
Silva, Giselle Aparecida Fagundes; Romero, Gustavo Adolfo Sierra; Cupolillo, Elisa; Yamashita, Ellen Priscila Gadelha; Silva, Adriano GomesIn the Brazilian Amazon, American tegumentary leishmaniasis (ATL) is endemic and presents a wide spectrum of clinical manifestations due, in part, to the circulation of at least seven Leishmaniaspecies. Few reports of Leishmania (Viannia) naiffiinfection suggest that its occurrence is uncommon and the reported cases present a benign clinical course and a good response to treatment. This study aimed to strengthe...
Specificity of the rapid rK39 antigen-based immunochromatographic test Kalazar ...
Molinet, Félix Javier León; Ampuero Vela, Julia Sonia; Costa, Rodrigo Diniz; Noronha, Elza Ferreira; Romero, Gustavo Adolfo SierraThe aim of this study was to evaluate the specificity of a rapid immunochromatographic test that was developed to detect antibodies against the rK39 antigen for the diagnosis of visceral leishmaniasis (VL). This evaluation was performed using sera from patients with a confirmed diagnosis of active cutaneous leishmaniasis. The sera from 272 patients with a confirmed diagnosis of localised cutaneous leishmaniasis...
Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of lo...
Ampuero Vela, Julia Sonia; Rios, Alexandre Pereira; Carranza Tamayo, César Omar; Romero, Gustavo Adolfo SierraThe positivities of two methods for the diagnosis of localised cutaneous leishmaniasis (CL) were estimated in 280 patients enrolled in a clinical trial. The trial was conducted in an endemic area of Leishmania (Viannia) braziliensis and trial participants were patients with skin ulcers and positive leishmanin skin tests. Patients underwent aspirative skin punctures of the ulcerated lesions and lymph nodes for i...
Polymerase chain reaction-based method for the identification of Leishmania (Vi...
Prestes, Suzane Ribeiro; Guerra, Jorge Augusto de Oliveira; Romero, Gustavo Adolfo Sierra; Magalhães, Laylah Kelre Costa; Santana, Rosa Amelia GonçalvesIntroduction: in the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmaniadeoxyribonucleic acid (DNA) within paraffin-embedded fragments of mucosal tissues, and characterizing the infecting parasite spe...
Sensitivity and specificity of parallel or serial serological testing for detec...
Arruda, Mauro Maciel de; Figueiredo, Fabiano Borges; Marcelino, Andreza Pain; Barbosa, José Ronaldo; Werneck, Guilherme Loureiro; Noronha, Elza FerreiraIn Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) as the screening and co...