4 documents found, page 1 of 1
Design of new hydrolyzed collagen-modified magnetic nanoparticles to capture pa...
Sande, Maria Georgina; Roque, Lúcia; Braga, Adelaide Correia; Marques, Márcia; Ferreira, Débora Carina Gonçalves Abreu; Saragliadis, AthanasiosEnrichment and diagnosis tools for pathogens currently available are time consuming, thus the development of fast and highly sensitive alternatives is desirable. In this study, a novel approach was described that enables selective capture of bacteria expressing hydrolyzed collagen-binding adhesins with hydrolyzed collagen-coated magnetic nanoparticles (MNPs). This platform could be useful to shorten the time ne...
Collagen-coated magnetic nanoparticles to capture pathogens
Sande, Maria; Roque, Lúcia; Marques, Márcia; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Braga, Adelaide Correia; Ramada, DiogoBackground The conventional method of diagnosing bacterial infections is based on microbial culture, however this method can take several hours or even days and lacks sensitivity. In the past few decades, the use of immunological and molecular techniques such as ELISA, PCR and mass spectrometry are used to diagnose infections. While these methods can be highly sensitive and reliable, they often require sample p...
Collagen-coated magnetic nanoparticles to capture pathogens from biological sam...
Sande, Maria; Roque, Lúcia; Marques, Márcia; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Braga, Adelaide; Ramada, David; Silva, C.Conventional methods of diagnosing bacterial infections such as microbial culture and molecular techniques, while highly sensitive, rely on expensive equipment and highly skilled operators. There is a need for affordable and portable diagnostic systems which are simple to operate while preserving reliability. Pre-enrichment of bacteria present in a sample coupled with subsequent bacterial identification steps c...
TP53 germline mutations in Portugal and genetic modifiers of age at cancer onset
Pinto, Carla; Veiga, Isabel; Pinheiro, Manuela; Peixoto, Ana; Pinto, Armando; Lopes, José M.; Reis, R. M.; Baptista, Manuela; Roque, LúciaThe Li-Fraumeni syndrome (LFS) is a rare, autosomal dominant disease caused by TP53 germline mutations. This study aimed to characterize the TP53 mutational spectrum in patients suspected to have LFS in Portugal and to evaluate the influence of the MDM2-SNP309 and TP53-72Arg variants and of telomere length on age of tumor onset. Probands were primarily selected using the classical LFS criteria (two cases) or th...