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Serum-Free Suspension Adaptation of HEK-293T Cells: Basis for Large-Scale Bioph...
Lomba,Ana Luiza Oliveira; Tirapelle,Mariane Cariati; Biaggio,Rafael Tagé; Abreu-Neto,Mário Soares; Covas,Dimas Tadeu; Picanço-Castro,VirginiaAbstract Human Embryonic Kidney 293T cells (HEK-293T) are the most common host for viral vector production and are also widely employed for recombinant protein production. These cells are typically cultured in monolayer (adherent culture) using culture medium containing fetal bovine serum (FBS), which impairs batch-to-batch reproducibility and scale-up. The adaptation of adherent cell culture to suspension cult...
Date: 2021
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Origin: Oasisbr