7 documents found, page 1 of 1
Impact of malting process on the amino acid profile of buckwheat seeds
Motta, Carla; Matos, Ana Sofia; Rosa, Filipa; Cabral, Izunildo; Castanheira, Isabel; Moreira, TiagoBuckwheat is a gluten-free pseudocereal recognised as an important source of protein. Malting is a form of processing that can improve the protein quality of food. The aim of this study is the optimization of malting conditions on protein content and its essential amino acids on buckwheat seeds. To achieve this goal, soaking time was optimised to a moisture content of 50% (2h) and the drying time to a moisture ...
Bioactivity screening of marine cyanobacteria for the isolation of novel compou...
Moreira, Gabriela A.; Rosa, Filipa; Vasconcelos, Vítor; Urbatzka, RalphNonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease and effective therapeutics has been widely study. Cyanobacteria, previously known as blue-green algae, are known to produce many secondary metabolites including toxins, with applications to the pharmaceutical industry. The aim of this study is to investigate the effect of extract from cyanobacteria to reduce lipid accumulation in a model...
Metabolic profiling of recombinant Escherichia coli cultivations based on high-t...
Sales, Kevin C.; Rosa, Filipa; Ribeiro Da Cunha, Bernardo; Sampaio, Pedro; B. Lopes, Marta; Calado, CecíliaEscherichia coli is one of the most used host microorganism for the production of recombinant products, such as heterologous proteins and plasmids. However, genetic, physiological and environmental factors influence the plasmid replication and cloned gene expression in a highly complex way. To control and optimize the recombinant expression system performance, it is very important to understand this complexity. ...
Monitoring the ex-vivo expansion of human mesenchymal stem/stromal cells in xen...
Rosa, Filipa; Sales, Kevin C.; Carmelo, Joana G.; Fernandes-Platzgummer, Ana; Silva, Cláudia L. da; Lopes, Marta B.; Calado, CecíliaHuman mesenchymal stem/stromal cells (MSCs) have received considerable attention in the field of cell-based therapies due to their high differentiation potential and ability to modulate immune responses. However, since these cells can only be isolated in very low quantities, successful realization of these therapies requires MSCs ex-vivo expansion to achieve relevant cell doses. The metabolic activity is one of...
A comprehensive high-throughput FTIR spectroscopy-based method for evaluating t...
Rosa, Filipa; Sales, Kevin C.; Cunha, Bernardo R.; Couto, Andreia; Lopes, Marta B.; Calado, CecíliaReporter genes are routinely used in every laboratory for molecular and cellular biology for studying heterologous gene expression and general cellular biological mechanisms, such as transfection processes. Although well characterized and broadly implemented, reporter genes present serious limitations, either by involving time-consuming procedures or by presenting possible side effects on the expression of the ...
Molecular fingerprint of human gastric cell line infected by Helicobacter pylori
Sampaio, Pedro; Ribeiro Da Cunha, Bernardo; Rosa, Filipa; Sales, Kevin; Lopes, Marta; Calado, CecíliaHelicobacter pylori infection represents a serious health problem, given its association with serious gastric diseases as gastric ulcers, cancer and MALT lymphoma. Currently no vaccine exists and antibiotic-based eradication therapy is already failing in more than 20% of cases. To increase the knowledge on the infection process diverse gastric cell lines, e.g. the adenocarcinona gastric (AGS) cell line, are rou...
In Situ Near-Infrared (NIR) Versus High-Throughput Mid- Infrared (MIR) Spectros...
Sales, Kevin C.; Rosa, Filipa; Sampaio, Pedro N.; Fonseca, LuÍs P.; B. Lopes, Marta; Calado, CecíliaThe development of biopharmaceutical manufacturing processes presents critical constraints, with the major constraint being that living cells synthesize these molecules, presenting inherent behavior variability due to their high sensitivity to small fluctuations in the cultivation environment. To speed up the development process and to control this critical manufacturing step, it is relevant to develop high-thr...