3 documents found, page 1 of 1
Myocardial RNA sequencing reveals new potential therapeutic targets in heart fa...
Inácio, José M.; Cristo, Fernando; Pinheiro, Miguel; Vasques-Nóvoa, Francisco; Saraiva, Francisca; Nunes, Mafalda M.; Rosas, Graça; Reis, AndreiaHeart failure with preserved ejection fraction (HFpEF) represents a global health challenge, with limited therapies proven to enhance patient outcomes. This makes the elucidation of disease mechanisms and the identification of novel potential therapeutic targets a priority. Here, we performed RNA sequencing on ventricular myocardial biopsies from patients with HFpEF, prospecting to discover distinctive transcri...
Generation and characterization of a human iPS cell line from a patient-related...
Pars, Selin; Cristo, Fernando; Inácio, José M.; Rosas, Graça; Carreira, Isabel Marques; Melo, Joana Barbosa; Mendes, Patrícia; Martins, Duarte SaraivaA DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited...
Generation of human iPSC line from a patient with laterality defects and associ...
Cristo, Fernando; Inácio, José M; Rosas, Graça; Carreira, Isabel Marques; Melo, Joana Barbosa; Almeida, Luís Pereira de; Mendes, PatríciaA human iPSC line was generated from exfoliated renal epithelial (ERE) cells of a patient affected with Congenital Heart Disease (CHD) and Laterality Defects carrying tshe variant p.R152H in the DAND5 gene. The transgene-free iPSCs were generated with the human OSKM transcription factor using the Sendai-virus reprogramming system. The established iPSC line had the specific heterozygous alteration, a stable kary...