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Shaping of Hepatic Ischemia/Reperfusion Events: The Crucial Role of Mitochondria
Teodoro, João S.; Silva, Rui T. da; Machado, Ivo; Panisello-Roselló, Arnau; Roselló-Catafau, Joan; Rolo, Anabela P.; Palmeira, Carlos M.Hepatic ischemia reperfusion injury (HIRI) is a major hurdle in many clinical scenarios, including liver resection and transplantation. Various studies and countless surgical events have led to the observation of a strong correlation between HIRI induced by liver transplantation and early allograft-dysfunction development. The detrimental impact of HIRI has driven the pursuit of new ways to alleviate its advers...
PEG35 as a Preconditioning Agent against Hypoxia/Reoxygenation Injury
Silva, Rui Teixeira da; Machado, Ivo F.; Teodoro, João S.; Panisello-Roselló, Arnau; Roselló-Catafau, Joan; Rolo, Anabela P.; Palmeira, Carlos M.Pharmacological conditioning is a protective strategy against ischemia/reperfusion injury, which occurs during liver resection and transplantation. Polyethylene glycols have shown multiple benefits in cell and organ preservation, including antioxidant capacity, edema prevention and membrane stabilization. Recently, polyethylene glycol 35 kDa (PEG35) preconditioning resulted in decreased hepatic injury and prote...
Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft P...
Bardallo, Raquel G.; Silva, Rui Teixeira da; Carbonell, Teresa; Folch-Puy, Emma; Palmeira, Carlos; Roselló-Catafau, Joan; Pirenne, Jacques; Adam, RenéThe total damage inflicted on the liver before transplantation is associated with several surgical manipulations, such as organ recovery, washout of the graft, cold conservation in organ preservation solutions (UW, Celsior, HTK, IGL-1), and rinsing of the organ before implantation. Polyethylene glycol 35 (PEG35) is the oncotic agent present in the IGL-1 solution, which is an alternative to UW and Celsior soluti...
Polyethylene Glycol 35 as a Perfusate Additive for Mitochondrial and Glycocalyx...
Panisello Rosello, Arnau; Silva, Rui Teixeira da; Castro, Carlos; Bardallo, Raquel G.; Calvo, Maria; Folch-Puy, Emma; Carbonell, TeresaOrgan transplantation is a multifactorial process in which proper graft preservation is a mandatory step for the success of the transplantation. Hypothermic preservation of abdominal organs is mostly based on the use of several commercial solutions, including UW, Celsior, HTK and IGL-1. The presence of the oncotic agents HES (in UW) and PEG35 (in IGL-1) characterize both solution compositions, while HTK and Cel...
Aldehyde Dehydrogenase 2 (ALDH2) in Rat Fatty Liver Cold Ischemia Injury
Panisello-Roselló, Arnau; Alva, Norma; Flores, Marta; Lopez, Alexandre; Castro Benítez, Carlos; Folch-Puy, Emma; Rolo, Anabela; Palmeira, CarlosInstitut George Lopez-1 (IGL-1) and Histidine-tryptophan-ketoglutarate (HTK) solutions are proposed as alternatives to UW (gold standard) in liver preservation. Their composition differs in terms of the presence/absence of oncotic agents such as HES or PEG, and is decisive for graft conservation before transplantation. This is especially so when fatty (steatotic) livers are used since these grafts are more vuln...
Cytoprotective Mechanisms in Fatty Liver Preservation against Cold Ischemia Inj...
Panisello-Roselló, Arnau; Verde, Eva; Lopez, Alexandre; Flores, Marta; Folch-Puy, Emma; Rolo, Anabela; Palmeira, Carlos; Hotter, GeorginaInstitute Goeorges Lopez 1 (IGL-1) and Histidine-Tryptophan-Ketoglutarate (HTK) preservation solutions are regularly used in clinical for liver transplantation besides University of Wisconsin (UW) solution and Celsior. Several clinical trials and experimental works have been carried out comparing all the solutions, however the comparative IGL-1 and HTK appraisals are poor; especially when they deal with the und...
GSK3β and VDAC Involvement in ER Stress and Apoptosis Modulation during Orthoto...
Zaouali, Mohamed; Panisello, Arnau; Lopez, Alexandre; Castro, Carlos; Folch, Emma; Carbonell, Teresa; Rolo, Anabela; Palmeira, CarlosWe investigated the involvement of glycogen synthase kinase-3β (GSK3β) and the voltage-dependent anion channel (VDAC) in livers subjected to cold ischemia-reperfusion injury (I/R) associated with orthotopic liver transplantation (OLT). Rat livers were preserved in University of Wisconsin (UW) and Institute Georges Lopez (IGL-1) solution, the latter enriched or not with trimetazidine, and then subjected to OLT. ...
PPARα Agonist WY-14643 Induces SIRT1 Activity in Rat Fatty Liver Ischemia-Reper...
Pantazi, Eirini; Folch-Puy, Emma; Bejaoui, Mohamed; Panisello, Arnau; Varela, Ana Teresa; Rolo, Anabela Pinto; Palmeira, Carlos M.Ischemia-reperfusion injury (IRI) remains a frequent complication in surgery, especially in case of steatotic livers that present decreased tolerance towards IRI. Apart from its major role in metabolism, activation of peroxisome proliferator-activated receptor α (PPARα) has been related with positive effects on IRI. In addition, the deacetylase enzyme sirtuin 1 (SIRT1) has recently emerged as a promising target...