7 documents found, page 1 of 1
Improvement of Mycobacterium tuberculosis detection in sputum using DNA extract...
Bello,Graziele Lima; Morais,Franciele Costa Leite; Wolf,Jonas Michel; Gehlen,Mirela; Soares,Tainá dos Santos; Halon,Maria Laura; Barcellos,Regina BonesAbstract Tuberculosis (TB) is one of the infectious diseases with high mortality in the world. DNA amplification techniques have been used to overcome barriers to the diagnosis of this disease. However, the success of these methodologies is highly dependent on the DNA obtained from the sample. This study was carried out to verify whether the DNA extracted by sonication (in house method) could yield suitable DNA...
Association between cervical lesion grade and micronucleus frequency in the Pap...
Bueno,Caroline Tanski; Silva,Cláudia Maria Dornelles da; Barcellos,Regina Bones; Silva,Juliana da; Santos,Carla Rossana dosThe aim of this study was to evaluate the association between the frequency of micronuclei (MN) and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the g...
Association between human papillomavirus (HPV) DNA and micronuclei in normal ce...
Cassel,Ana Paula Rebelo; Barcellos,Regina Bones; Silva,Cláudia Maria Dornelles da; Almeida,Sabrina Esteves de Matos; Rossetti,Maria Lucia RosaThe aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7...
Metabolic changes associated with antiretroviral therapy in HIV-positive patients
Almeida,Sabrina Esteves de Matos; Borges,Michele; Fiegenbaum,Marilu; Nunes,Cynara Carvalho; Rossetti,Maria Lucia RosaOBJECTIVE: To evaluate metabolic changes associated with highly active antiretroviral therapy (HAART) in HIV-positive patients, and to identify risk factors associated. METHODS: Retrospective study that included 110 HIV-positive patients who where on HAART in the city of Porto Alegre (Southern Brazil) between January 2003 and March 2004. Data on demographic variables, cigarette smoking, diabetes mellitus, chole...
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvem...
Cortez-Herrera,Elizabeth; Sperhacke,Rosa Dea; Becker,Daniela; Kritski,Afrânio; Zaha,Arnaldo; Rossetti,Maria Lucia RosaThe aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the ...
Análise molecular de cepas de Mycobacterium tuberculosis provenientes de um cen...
Borges,Michele; Cafrune,Patrícia Izquierdo; Possuelo,Lia Gonçalves; Valim,Andréia Rosane de Moura; Ribeiro,Marta Osório; Rossetti,Maria Lucia RosaINTRODUÇÃO: A tuberculose é uma doença antiga que ainda se mantém como um dos maiores males da humanidade no século XXI. Nas últimas décadas, o advento de novas tecnologias utilizando os conhecimentos de biologia molecular tem levado a um aumento na investigação da etiologia, detecção e epidemiologia da tuberculose. OBJETIVO: Avaliar o grau de similaridade entre as cepas de Mycobacterium tuberculosis provenient...
Avaliação de testes rápidos em microplacas usando indicadores de viabilidade ce...
Ribeiro,Marta Osório; Gomes,Marlei da Silva; Senna,Simone Gonçalves; Rossetti,Maria Lucia Rosa; Fonseca,Leila de SouzaINTRODUÇÃO: As taxas de resistência aos fármacos constituem um dos pilares da avaliação dos programas de controle da tuberculose. A demora na obtenção dos resultados, conseqüência da metodologia convencional utilizada, faz com que haja a necessidade de avaliação de novos testes, mais rápidos e menos onerosos. OBJETIVO: Comparar técnicas fenotípicas rápidas para determinação do perfil de susceptibilidade de M. t...