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Detection of Mycobacterium tuberculosis complex by nested polymerase chain reac...

Furini,Adriana Antônia da Cruz; Pedro,Heloisa da Silveira Paro; Rodrigues,Jean Francisco; Montenegro,Lilian Maria Lapa; Machado,Ricardo Luiz Dantas

OBJECTIVE: To compare the performance of nested polymerase chain reaction (NPCR) with that of cultures in the detection of the Mycobacterium tuberculosis complex in pulmonary and extrapulmonary specimens.METHODS: We analyzed 20 and 78 pulmonary and extrapulmonary specimens, respectively, of 67 hospitalized patients suspected of having tuberculosis. An automated microbial system was used for the identification o...

Date: 2013   |   Origin: Oasisbr

Concurrent dengue and malaria in the Amazon region

Santana,Vinícius dos Santos; Lavezzo,Lígia Carolina; Mondini,Adriano; Terzian,Ana Carolina Bernardes; Bronzoni,Roberta Vieira de Moraes

INTRODUCTION: The Amazon region has extensive forested areas and natural ecosystems, providing favorable conditions for the existence of innumerous arboviruses. Over 200 arboviruses have been isolated in Brazil and about 40 are associated with human disease. Four out of 40 are considered to be of public health importance in Brazil: Dengue viruses (1-4), Oropouche, Mayaro and Yellow Fever. Along with these virus...

Date: 2010   |   Origin: Oasisbr

Genetic relatedness among clinical strains of Stenotrophomonas maltophilia in t...

Almeida,Margarete Teresa Gottardo; Rubio,Fernando Gôngora; Garcia,Doroti Oliveira; Pavarino-Bertelli,Érika Cristina; Rossit,Andrea Regina Baptista

Stenotrophomonas maltophilia is a Gram-negative bacillus, which is becoming widely recognized as an important nosocomial pathogen. The main objective of this study was to evaluate the genetic relatedness, by random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of 86 clinical isolates of S. maltophilia (colonization 22, infection 64) obtained from 79 hospitalized patients, from dif...

Date: 2007   |   Origin: Oasisbr

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