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Purification and characterization of hyaluronic acid from chicken combs
Rosa,Claudia Severo da; Tovar,Ana Freire; Mourão,Paulo; Pereira,Ricardo; Barreto,Pedro; Beirão,Luiz HenriqueHyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. The umbilical cord and the chicken comb are some of the tissues richest in this polysaccharide. The profit from obtaining HA from the combs of slaughtered animals is particularly attractive. This work aimed to extract, purify, and characterize HA. The glycosaminoglycan concentration in the chicken comb was found to be about...
Atividade antioxidante do ácido hialurônico extraído da crista de frango
Rosa,Claudia Severo da; Hoelzel,Solange Cristina; Viera,Vanessa Bordin; Barreto,Pedro Manique; Beirão,Luiz HenriqueO ácido hialurônico (AH) é uma macromolécula com importância na área médica, na área farmacêutica e na indústria de cosméticos. O cordão umbilical e a crista de galo são os tecidos mais ricos neste polissacarídeo. O Brasil é um dos principais exportadores de frango do mundo; assim, o aproveitamento das cristas dos animais abatidos para a obtenção de AH se mostra particularmente atraente. Este trabalho teve como...
Effect of different levels of sodium chloride and glucose on fermentation of sa...
Espirito Santo,Milton Luiz Pinho; Lisboa,Cristiane; Alves,Fernanda Gonçalves; Martins,Daniela; Beirão,Luiz Henrique; Sant'Anna,Ernani SebastiãoLactobacillus sakei 2a is a bacteriocin producer strain. In this study, it's effects as a starter culture in the curing process of sardine (Sardinella brasiliensis) fillets were studied at different concentrations of NaCl (2, 4, 6%) and glucose (2, 4%). After 21 days of fermentation, the spoilage microorganisms population reached 9.7 Log10 CFU g-1 corresponding to 6% NaCl and 4% glucose. With no addition of glu...
Purification and characterisation of trypsin-like enzyme from the pyloric caeca...
Beirão,Luiz Henrique; Mackie,Ian Mckintoch; Teixeira,Evanilda; Damian,CésarA trypsin -like enzyme from the pyloric caeca of cod (Gadus morhua) was purified by affinity chromatography on CHOM Sepharose 4B. Some characteristics were established by its catalytic activity on T.A.M.E., typical enzyme substrate, and serine protease inhibitors. The enzyme had an isoelectric point of 5.30 and 5.89 and was very similar in amino acid composition to bovine trypsin, but differed in having a highe...