2 documents found, page 1 of 1
Neuronal cell-based high-throughput screen for enhancers of mitochondrial funct...
Naia, Luana Carvalho; Pinho, Catarina M.; Dentoni, Giacomo; Liu, Jianping; Leal, Nuno Santos; Ferreira, Duarte M. S.; Schreiner, BernadetteBackground: Mitochondrial dysfunction is a common feature of aging, neurodegeneration, and metabolic diseases. Hence, mitotherapeutics may be valuable disease modifiers for a large number of conditions. In this study, we have set up a large-scale screening platform for mitochondrial-based modulators with promising therapeutic potential. Results: Using differentiated human neuroblastoma cells, we screened 1200 F...
Bioenergetic cues shift FXR splicing towards FXR alpha 2 to modulate hepatic li...
Correia, Jorge; Massart, Julie; de Boer, Jan Freark; Porsmyr-Palmertz, Margareta; Martinez-Redondo, Vicente; Agudelo, Leandro Z.; Sinha, IndranilObjective: Farnesoid X receptor (FXR) plays a prominent role in hepatic lipid metabolism. The FXR gene encodes four proteins with structural differences suggestive of discrete biological functions about which little is known. Methods: We expressed each FXR variant in primary hepatocytes and evaluated global gene expression, lipid profile, and metabolic fluxes. Gene delivery of FXR variants to Fxr(-/-) mouse liv...