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Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in t...

Lamy, Elsa; Rubio, Camila; Carreira, Laura; Capela e Silva, Fernando; Martinez-Subiela, Silvia; Tecles, Fernando; Lopez-Jornet, Pia; Céron, Jose

The present study aims to assess the effects of thermal and chemical inactivating procedures, that can be used for SARS-CoV-2 inactivation, on different salivary analytes. SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile and a panel of 25 specific biomarkers of oxidative status, stress, metabolism and tissue damage were evaluated in samples subjected to different treatments: thermal (65 °C or 9...


Changes in saliva analytes in dairy cows during peripartum: A pilot study

Contreras-Aguilar, Maria Dolores; Vallejo-Mateo, P.J.; Lamy, Elsa; Escribano, Damian; Ceron, Jose Joaquim; Tecles, Fernando; Rubio, Camila

This pilot study aimed to study the possible changes in a profile of 26 salivary analytes in thirteen healthy dairy cows along their peripartum period. Analytes associated with the stress (salivary cortisol, salivary alpha-amylase, butyrylcholinesterase, and total esterase), inflammation (adenosine deaminase), oxidative status (total antioxidant capacity and the advanced oxidation protein products), and general...


Analytical validation of an automated assay for the measurement of adenosine de...

Franco-Martinéz, Lorena; Tecles, Fernando; Torres-Cantero, A.; Bernal, E.; San-Lázaro, I.; Alcaraz, M.J.; Vicente-Romero, M.R.; Lamy, Elsa

Objectives The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection. Methods The validation, in addition to the evaluation of precision and accuracy, included the ...


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