5 documents found, page 1 of 1
A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transep...
Hevia, Arancha; Ruas-Madiedo, Patricia; Faria, Miguel Angelo; Petit, Valérie; Alves, Bruna; Alvito, Paula; Arranz, Elena; Bastiaan-Net, ShannaAssessing nutrient bioavailability is complex, as the process involves multiple digestion steps, several cellular environments, and regulatory-metabolic mechanisms. Several in vitro models of different physiological relevance are used to study nutrient absorption, providing significant challenges in data evaluation. However, such in vitro models are needed for mechanistic studies as well as to screen for biolog...
Standardization of in vitro digestibility and DIAAS method based on the static ...
Egger, Lotti; Sousa, Raquel; Recio, Isidra; Abbühl, Lychou; Brügger, Cédric; Hernández, Marta Santos; Macierzanka, Adam; Szumala, PatrycjaBackground: The FAO recommends the digestible indispensable amino acid score (DIAAS) as the measure for protein quality, for which the true ileal digestibility needs to be assessed in humans or pigs. However, due to high costs and ethical concerns, the FAO strongly encourages as well the development of validated in vitro methods, which complement the in vivo experiments. Method: Recently, an in vitro workflow, ...
Computational approach to the systematic prediction of glycolytic abilities: lo...
Blanco, Guillermo; Sanchez, Borja; Ruiz, Lorena; Fdez-Riverola, Florentino; Margolles, Abelardo; Lourenço, AnáliaGlycoside hydrolases are responsible for the enzymatic deconstruction of complex carbohydrates. Most of the families are known to conserve the catalytic machinery and molecular mechanisms. This work introduces a new method to predict glycolytic abilities in sequenced genomes and thus, gain a better understanding of how to target specific carbohydrates and identify potentially interesting sources of specialised ...
Revisiting the metabolic capabilities of Bifidobacterium longum susbp. longum a...
Blanco, Guillermo; Ruiz, Lorena; Tamés, Hector; Ruas-Madiedo, Patricia; Fdez-Riverola, Florentino; Sánchez, Borja; Lourenço, Anália; Margolles, AbelardoBifidobacteria are among the most abundant microorganisms inhabiting the intestine of humans and many animals. Within the genus Bifidobacterium, several beneficial effects have been attributed to strains belonging to the subspecies Bifidobacterium longum subsp. longum and Bifidobacterium longum subsp. infantis, which are often found in infants and adults. The increasing numbers of sequenced genomes belonging to...
Tackling probiotic and gut microbiota functionality through proteomics
Ruiz, Lorena; Hidalgo, Claudio; Blanco-Míguez, Aitor; Lourenço, Anália; Sánchez, Borja; Margolles, AbelardoAbstract Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Many strains exert their beneficial effects after transiently colonizing the human gut, where they interact with the rest of the intestinal microorganisms and with the host mucosa. Indeed the human gut harbours a huge number of microorganisms also known as gut microbiota. Imbalances in th...