8 documents found, page 1 of 1
Automatic and renewable micro-solid-phase extraction based on bead injection la...
Fernandes, Sara; Barreiros, Luísa; Sá, Paula; Miró, Manuel; Segundo, Marcela A.An automatic micro-solid-phase extraction (μSPE) method using on-line renewable sorbent beads followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was established for the determination of tranexamic acid (TXA) in urine. The μSPE method was based on the bead injection (BI) concept combined with the mesofluidic lab-on-valve (LOV) platform. All steps of the μSPE–BI–LOV were implemented by computer...
A ampliação dos danos indenizáveis frente aos novos padrões da responsabilidade...
Sá, PaulaCinge-se o presente trabalho ao estudo dos novos padrões adotados pelo instituto da responsabilidade civil e como estes novos padrões propiciaram o surgimento de novos danos indenizáveis. Nos últimos anos, o instituto da responsabilidade civil vem se amoldando a novos padrões e superando o arcaico modelo subjetivista e patrimonialista puramente adotado até o final do século XIX. Ocorre que, como consequência da...
Acetonitrile adducts of tranexamic acid as sensitive ions for quantification at...
Silva, Eduarda M. P.; Barreiros, Luísa; Fernandes, Sara R.; Sá, Paula; Ramalho, João P. Prates; Segundo, Marcela A.The quantitative analysis of pharmaceuticals in biomatrices by liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is often hampered by adduct formation. The use of the molecular ion resulting from solvent adducts for quantification is uncommon, even if formed in high abundance. In this work, we propose the use of a protonated acetonitrile adduct for the quantitati...
Determination of tranexamic acid in human plasma by UHPLC coupled with tandem m...
Barreiros, Luisa; Amoreira, Júlia L.; Machado, Sandia; Fernandes, Sara R.; Silva, Eduarda M.P.; Sá, Paula; Kietaibl, Sibylle; Segundo, Marcela A.Tranexamic acid (TXA) is an antifibrinolytic drug, with the ability to inhibit lysine binding at plasminogen receptors, used in adult trauma patients with on-going or at risk of significant haemorrhage. To understand the pharmacokinetics and pharmacodynamics of this drug in variable age groups undergoing surgeries with high blood loss, effective methods for determination of TXA in biological samples at sub-μg m...
Quantification of tranexamic acid in human plasma: development and validation o...
Barreiros, Luisa; Amoreira, Júlia L.; Machado, Sandia; Fernandes, Sara R.; Silva, Eduarda M. P.; Sá, Paula; Kietaibl, Sibylle; Segundo, Marcela A.Tranexamic acid (TXA), an antifibrinolytic drug with the ability to inhibit lysine binding at plasminogen receptors, can be used in different settings such as trauma, cardiac surgery, major orthopedic surgery, obstetric when perioperative bleeding is concerned [1]. Effective methods for determination of TXA in biological samples are still required to understand the pharmacokinetics and pharmacodynamics of this ...
Analytical methods for quantification of tranexamic acid in biological fluids: ...
Silva, Eduarda M.P.; Barreiros, Luisa; Sá, Paula; Afonso, Carlos; Kozek-Langenecker, Sibylle; Segundo, Marcela A.Tranexamic acid (TXA) is a synthetic derivative of the amino acid lysine with antifibrinolytic properties. There is still a lack of pharmacokinetic and pharmacodynamic data concerning variable age groups undergoing surgeries with high blood loss. The optimum dose and administration schedules of TXA are still subject of research, aiming at a safe inhibition of fibrinolysis in the perioperative period. Hence, eff...
Spontaneous one-lung ventilation increases the lung inflammatory response : an ...
Machado, Humberto S.; Sá, Paula; Nunes, Catarina S.; Couceiro, António; Silva, Álvaro Moreira da; Águas, ArturStudy objective: The purpose of this study was to investigate if spontaneous one-lung ventilation would induce any type of inflammatory lung response when compared to spontaneous two-lung ventilation and its intensity, by quantification of inflammatory cells in lung histology at the end of the procedure. Design: In vivo prospective randomised animal study Setting: University research laboratory Subjects: New Ze...
Increased lung inflammation with oxygen supplementation in tracheotomized spont...
Machado, Humberto S.; Nunes, Catarina S.; Sá, Paula; Couceiro, António; Silva, Álvaro Moreira da; Águas, ArturBackground Mechanical ventilation is a well–known trigger for lung inflammation. Research focuses on tidal volume reduction to prevent ventilator-induced lung injury. Mechanical ventilation is usually applied with higher than physiological oxygen fractions. The purpose of this study was to investigate the after effect of oxygen supplementation during a spontaneous ventilation set up, in order to avoid the infla...