4 documents found, page 1 of 1
Highly fluorescent 2-amino-6-cyanopurines as new bioimaging tools for studying ...
Gonçalves, João N. D.; Gonçalves, Jorge M.; Rodrigues, L. R.; Coutinho, Paulo J. G.; Sá, Paulo Correia de; Castanheira, Elisabete M. S.Fluorescent nucleobase analogues (FNAs) have been extensively used for scientific and biomedical purposes owing to their relevant photophysical properties. In this study, five newly synthesized 2-amino-6-cyanopurines were evaluated for their fluorescent probing and imaging capabilities using Saccharomyces cerevisiae as model organism. The new compounds, which were synthesized using highly efficient cost-effecti...
Morphofunctional characterization of the ileum in a type 1 diabetes rat model 7
Henriques, Vanessa Mendes; Monteiro, Salomé Gonçalves; Vieira, Filipa Quintela; Sá, Paulo Correia de; Araújo, Margarida DuarteDiabetes mellitus (DM) is a metabolic disorder that affects 1 in 12 people with high mortality and morbidity. Knowing that about 76% of diabetic patients have gastrointestinal complications, we decided to characterize morphologically and functionally the ileum of a rat (Rattus norvegicus, Wistar) model that mimics type 1 DM. Diabetes was chemically induced by a single intraperitoneal injection of streptozotocin...
Diabetic rats lose A2A receptor-mediated facilitation of ileal myenteric cholin...
Monteiro, Salomé Gonçalves; Cardoso, Maria Teresa Magalhães; Ferreirinha, Fátima; Henriques, Vanessa Mendes; Sá, Paulo Correia deEnteric dysmotility is a long-term complication of Diabetes mellitus that causes significant discomfort in 76% of diabetic outpatients. Knowing that purines may be involved in synaptic transmission modifications in the CNS of diabetic rats, we decided to investigate if purinergic dysfunction could also play a role in diabetic enteric neuropathy in rats.
Ecto-AMP deaminase blunts the ATP-derived adenosine A2A receptor facilitation o...
Cardoso, M. Teresa Magalhães; Pereira, M. Fátima; Oliveira, Laura; Ribeiro, J. A.; Cunha, Rodrigo A.; Sá, Paulo Correia deAt synapses, ATP is released and metabolised through ecto-nucleotidases forming adenosine, which modulates neurotransmitter release through inhibitory A1 or facilitatory A2A receptors, according to the amounts of extracellular adenosine. Neuromuscular junctions possess an ecto-AMP deaminase that can dissociate extracellular ATP catabolism from adenosine formation. In this study we have investigated the pattern ...