6 documents found, page 1 of 1
Applicability of flow cytometry γH2AX assay in population studies: suitability ...
Laffon, B; Sánchez-Flores, M; Fernández-Bertólez, N; Pásaro, E; Valdiglesias, VPhosphorylation of H2AX histone (γH2AX) represents an early event in the DNA damage response against double-strand breaks (DSB); hence, its measurement provides a surrogate biomarker of DSB. Recently, we reported initial steps in the standardization of γH2AX assay in peripheral blood leukocytes (PBL), addressing the possibility of using cryopreserved samples, and the need of phytohaemagglutinin (PHA) stimulatio...
Low Vitamin D Levels and Frailty Status in Older Adults: A Systematic Review an...
Marcos-Pérez, D; Sánchez-Flores, M; Proietti, S; Bonassi, S; Costa, S; Teixeira, JP; Fernández-Tajes, J; Pásaro, E; Valdiglesias, V; Laffon, BSerum vitamin D deficiency is widespread among older adults and is a potential modifiable risk factor for frailty. Moreover, frailty has been suggested as an intermediate step in the association between low levels of vitamin D and mortality. Hence, we conducted a systematic review of the literature and meta-analysis to test the possible association of low concentrations of serum 25-hydroxyvitamin D (25(OH)D), a...
The impact of comet assay data normalization in human biomonitoring studies out...
Esteves, F; Amaro, R; Silva, S; Sánchez-Flores, M; Teixeira, JP; Costa, CThe comet assay has been extensively used in biomonitoring studies. To avoid intra-experimental variability, the incorporation of assay controls in each work session for data normalization has been suggested by some authors but has never been thoroughly analyzed. The aim of this study was to address the impact of data normalization in the results of a biomonitoring study using different normalization models. Hu...
Association of inflammatory mediators with frailty status in older adults: resu...
Marcos-Pérez, D; Sánchez-Flores, M; Proietti, S; Bonassi, S; Costa, S; Teixeira, JP; Fernández-Tajes, J; Pásaro, E; Laffon, B; Valdiglesias, VFrailty is a geriatric syndrome defined as a status of extreme vulnerability to stressors, leading to a higher risk of negative health-related outcomes. “Inflammaging”, an age-related state of low-grade chronic inflammation, is characterized by an increased concentration of pro-inflammatory cytokines and acute phase proteins. Inflammaging has been postulated as an underlying mechanism of frailty, and several st...
Exploring Genetic Outcomes as Frailty Biomarkers
Valdiglesias, V; Sánchez-Flores, M; Marcos-Pérez, D; Lorenzo-López, L; Maseda, A; Millán-Calenti, JC; Pásaro, E; Laffon, BFrailty has emerged as a reliable measure of the aging process. Because the early detection of frailty is crucial to prevent or even revert it, the use of biomarkers would allow an earlier and more objective identification of frail individuals. To improve the understanding of the biological features associated with frailty as well as to explore different biomarkers for its early identification, several genetic ...
Oxidative stress, genomic features and DNA repair in frail elderly: a systemati...
Sánchez-Flores, M; MArcos-Pérez, D; Costa, S; Teixeira, JP; Bonassi, S; Pásaro, E; Laffon, B; Valdiglesias, VFrailty is an emerging geriatric syndrome characterized by higher vulnerability to stressors, with an increased risk of adverse health outcomes such as mortality, morbidity, disability, hospitalization, and institutionalization. Although it is generally recognized to have a biological basis, no particular biological trait has been consistently associated to frailty status so far. In this work, epidemiological s...