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Kinetics of IFN-gamma, TNF-alpha, IL-10 and IL-4 production by mononuclear cell...

Sadahiro,Aya; Diogo,Constancia Lima; Oshiro,Telma Miyuki; Shikanai-Yasuda,Maria Aparecida

We analyzed the kinetics of cytokine production by mononuclear cells from 17 patients who had been treated for paracoccidioidomycosis, using the stimulus of gp43 peptide groups (43kDa glycoprotein of Paracoccidioides brasiliensis) at 0.1 and 1µM, gp43 (1µg/ml) and crude Paracoccidioides brasiliensis antigen (PbAg; 75µg/ml). IFN-gamma production was a maximum at 144 hours in relation to the G2 and G8 peptide gro...

Date: 2007   |   Origin: Oasisbr

HLA in Brazilian Ashkenazic Jews with chronic dermatophytosis caused by Trichop...

Sadahiro,Aya; Moraes,José Roberto Feresin; Moraes,Maria Elisa Hue; Romero,Matilde; Gouvea,Nancy Alves de Lima; Gouvea,Celso José

The frequency of HLA (Human Leucocyte Antigens) was analyzed in 25 non-consanguineous Brazilian Ashkenazic Jews, resident in the city of São Paulo, Brazil, suffering from chronic dermatophytosis caused by T. rubrum, and in 25 non-infected individuals belonging to the same ethnic group. Statistically significant values (p<0.05) were observed for HLA-B14 associated with resistance to chronic dermatophytosis and H...

Date: 2004   |   Origin: Oasisbr

PCR in the diagnosis of cutaneous tuberculosis

Ogusku,Mauricio Morishi; Sadahiro,Aya; Hirata,Mário Hiroyuki; Hirata,Rosário D. C.; Zaitz,Clarisse; Salem,Júlia Ignez

Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated(with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH)biopsies tissues samples stored at -20ºC. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% th...

Date: 2003   |   Origin: Oasisbr

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