10 documents found, page 1 of 1
Profiling of urinary extracellular vesicle protein signatures from patients wit...
Bernardino, R; Carvalho, AS; Hall, MJ; Alves, L; Leão, R; Sayyid, R; Pereira, H; Beck, HC; Campos-Pinheiro, L; Henrique, R; Fleshner, N; Matthiesen, RPrognostic tests and treatment approaches for optimized clinical care of prostatic neoplasms are an unmet need. Prostate cancer (PCa) and derived extracellular vesicles (EVs) proteome changes occur during initiation and progression of the disease. PCa tissue proteome has been previously characterized, but screening of tissue samples constitutes an invasive procedure. Consequently, we focused this study on liqui...
Assessment of a Large-Scale Unbiased Malignant Pleural Effusion Proteomics Stud...
Zahedi, S; Carvalho, AS; Ejtehadifar, M; Beck, HC; Rei, N; Luís, A; Borralho, P; Bugalho, A; Matthiesen, RBackground: Pleural effusion (PE) is common in advanced-stage lung cancer patients and is related to poor prognosis. Identification of cancer cells is the standard method for the diagnosis of a malignant PE (MPE). However, it only has moderate sensitivity. Thus, more sensitive diagnostic tools are urgently needed. Methods: The present study aimed to discover potential protein targets to distinguish malignant pl...
Comparative analysis of the bronchoalveolar microbiome in Portuguese patients w...
Seixas, S; Kolbe, AR; Gomes, S; Sucena, M; Sousa, C; Vaz Rodrigues, L; Teixeira, G; Pinto, P; Tavares de Abreu, T; Bárbara, C; Semedo, J; Mota, LThe lung is inhabited by a diverse microbiome that originates from the oropharynx by a mechanism of micro-aspiration. Its bacterial biomass is usually low; however, this condition shifts in lung cancer (LC), chronic obstructive pulmonary disease (COPD) and interstitial lung disease (ILD). These chronic lung disorders (CLD) may coexist in the same patient as comorbidities and share common risk factors, among whi...
Extracellular Vesicle Proteome in Prostate Cancer: A Comparative Analysis of Ma...
Bernardino, RM; Leão, R; Henrique, R; Pinheiro, LC; Kumar, P; Suravajhala, P; Beck, HC; Carvalho, AS; Matthiesen, RMolecular diagnostics based on discovery research holds the promise of improving screening methods for prostate cancer (PCa). Furthermore, the congregated information prompts the question whether the urinary extracellular vesicles (uEV) proteome has been thoroughly explored, especially at the proteome level. In fact, most extracellular vesicles (EV) based biomarker studies have mainly targeted plasma or serum. ...
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of ad...
Carvalho, AS; Moraes, MCS; Na, CH; Fierro-Monti, I; Henriques, A; Zahedi, S; Bodo, C; Tranfield, EM; Sousa, AL; Farinho, A; Rodrigues, LV; Pinto, PAcellular bronchoalveolar lavage (BAL) proteomics can partially separate lung cancer from non-lung cancer patients based on principal component analysis and multivariate analysis. Furthermore, the variance in the proteomics data sets is correlated mainly with lung cancer status and, to a lesser extent, smoking status and gender. Despite these advances BAL small and large extracellular vehicles (EVs) proteomes r...
Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer ...
Maia, J; Otake, AH; Poças, J; Carvalho, AS; Beck, HC; Magalhães, A; Matthiesen, R; Strano Moraes, MC; Costa-Silva, BPancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation of CD11b+ bone marrow (BM) cells, which support PC liver metastasis. We here show that PC-EVs also bind to CD11b+ BM c...
Profiling of lung microbiota discloses differences in adenocarcinoma and squamo...
Gomes, S; Cavadas, B; Ferreira, JC; Marques, PI; Monteiro, C; Sucena, M; Sousa, C; Rodrigues, LV; Teixeira, G; Pinto, P; Abreu, TT; Bárbara, CThe lung is a complex ecosystem of host cells and microbes often disrupted in pathological conditions. Although bacteria have been hypothesized as agents of carcinogenesis, little is known about microbiota profile of the most prevalent cancer subtypes: adenocarcinoma (ADC) and squamous cell carcinoma (SCC). To characterize lung cancer (LC) microbiota a first a screening was performed through a pooled sequencing...
Pharmacogenomics, CYP2D6, and tamoxifen: A survey of the reasons sustaining eur...
Reis, SS; Carvalho, AS; Fernandes, RTamoxifen is a drug that is often used in the clinical management of breast cancer. CYP2D6 is a key metabolizing enzyme that is involved in the conversion of tamoxifen to its active drug metabolites. CYP2D6 has several alleles that metabolize tamoxifen and other drugs at different rates that can alter therapeutic impact, a characteristic that renders it one of the most studied enzymes in the field of pharmacoge...
HIV and Coronary Disease - When Secondary Prevention Is Insufficient
Carvalho, AS; Osório Valente, R; Almeida-Morais, L; Daniel, P; Sá Carvalho, R; Ferreira, L; Cruz Ferreira, RHighly active antiretroviral therapy (HAART) has created a new paradigm for human immunodeficiency virus (HIV)-infected patients, but their increased risk for coronary disease is well documented. We present the case of a 57-year-old man, co-infected with HIV-2 and hepatitis B virus, adequately controlled and with insulin-treated type 2 diabetes and dyslipidemia, who was admitted with non-ST elevation acute myoc...
Helicobacter pylori cag pathogenicity island-positive strains induce syndecan-4...
Magalhães, A; Marcos, NT; Carvalho, AS; David, L; Figueiredo, C; Bastos, J; David, G; Reis, CAHelicobacter pylori is recognized as the main cause of gastritis and is associated with gastric carcinogenesis. Syndecan-4 represents the major source of heparan sulfate (HS) in the gastric cells. HS proteoglycans expressed on the cell surface constitute targets for H. pylori at the early stage of infection. The aim of this study was to determine whether H. pylori induction of syndecan-4 expression is affected ...