55 documents found, page 1 of 6
Silencing of FRO1 gene affects iron homeostasis and nutrient balance in tomato ...
Gama, Florinda; Saavedra, Teresa; Dandlen, Susana; García‐Caparrós, Pedro; de Varennes, Amarilis; Nolasco, Gustavo; Correia, Pedro JoséBackgroundIron chlorosis is an abiotic stress of worldwide importance affecting several agronomic crops. It is important to understand how plants maintain nutrient homeostasis under Fe deficiency and recovery. AimsWe used the virus-induced gene silencing (VIGS) method to elucidate the role of the FRO1 gene in tomato plants and identify the impact on regulation of the root ferric-chelate reductase (FCR) activity...
Tomato spotted wilt virus genes expressed in antisense orientation and their ab...
Pires, Vanessa; Dandlen, Susan; Nolasco, Gustavo; Felix, Maria; Materatski, Patrick; Varanda, Carla; Marques, NataliaTomato spotted wilt virus (TSWV) is a member of the Tospoviridae family and is ranked among the top ten economically important viruses in the world. The genome of TSWV consists of three linear negative-sense or ambisense RNA segments, denoted as segments L, M and S. Segment S RNA encodes the silencing suppressor NSs, and the nucleocapsid protein N. Segment M RNA encodes the cell-to-cell movement protein NSm and...
Development of OMMV as a VIGS vector for plant protection
Varanda, Carla; Felix, Maria; Materatski, Patrick; Campos, Maria; Patanita, Mariana; Marques, Natalia; Clara, Maria; Nolasco, GustavoBackground Viruses are responsible for several important plant diseases, however, they have also been used in biotechnology with different purposes. Virus induced gene silencing (VIGS) allows specific silencing of foreign genes that can be inserted in a viral vector and then inoculated in plants. When a sequence of a foreign gene is introduced in a VIGS vector, the plant infected with this vector will be signal...
Can bicarbonate enhance the performance of carob seedlings grown in nutrient so...
Gama, Florinda; Correia, Pedro José; Saavedra, T.; Dandlen, Susana; de Varennes, Amarilis; Nolasco, Gustavo; Pestana, MaribelaThe aim of this work was to assess the effect of bicarbonate (Bic) ion on the nutritional status and performance of carob-tree seedlings, a species that normally grows in calcareous soil without exhibiting iron chlorosis symptoms. Seedlings were previously grown in nutrient solution with a small concentration of Fe (0.5-1 mu M) to induce a moderate chlorosis. Afterwards, two experiments were established: in exp...
Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, a...
Varanda, Carla; Materatski, Patrick; Campos, Maria Doroteia; Clara, Maria Ivone; Nolasco, Gustavo; Félix, Maria do RosárioRNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this mechanism. In this study, the genome of the Olive mild mosaic virus (OMMV) was screened for silencing suppressors. The full OMMV cDNA and 5 OMMV open reading frames (ORFs) were cloned into the Gateway binary vector pK7WG2, transformed into Agrobacterium tumefaciens, and agroinfiltrated into N....
OLIVE MILD MOSAIC VIRUS CP AND P6 ARE SUPPRESSORS OF RNA SILENCING AND THEIR SI...
Varanda, Carla; Materatski, Patrick; Campos, Maria Doroteia; Nolasco, Gustavo; Clara, Maria Ivone; Félix, Maria do RosárioRNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this mechanism. Here the genome of Olive mild mosaic virus (OMMV) was screened for silencing suppressors in the herbaceous host Nicotiana benthamiana 16C using a green fluorescent based transient suppression assay. The full OMMV cDNA and 5 different OMMV open reading frames (ORFs) were cloned into ...
Vegetation drives assemblages of entomopathogenic nematodes and other soil orga...
Campos-Herrera, Raquel; Blanco-Pérez, Rubén; Bueno-Pallero, Francisco Ángel; Duarte, Amílcar; Nolasco, Gustavo; Sommer, Ralf J.Entomopathogenic nematodes (EPNs) are widely distributed in soils throughout the world. Their activity as biological control agents is modulated by abiotic and biotic factors (e.g. soil type, climatic fluctuation and natural enemies). We sought to identify soil properties in a Mediterranean region, which might be managed to enhance biological control agents’ services provided by EPNs. We hypothesized that respon...
Co infection of OMMV and OLV-1 enhances symptoms and increases both viruses acc...
Varanda, C. M.; Materatski, P.; Campos, M. D.; Clara, M. I.; Marques, N T.; Nolasco, Gustavo; Felix, M. R.Previous extensive field surveys in olive orchards have revealed high levels of Olive mild mosaic virus (OMMV) and Olive latent virus 1 (OLV-1), frequently appearing in mixed infections. These viruses belong to genus Alphanecrovirus and their RNA dependent RNA polymerase (RdRp),as well as their p6 and p8 amino acid sequences share over 87% identity.
Correction to: Can bicarbonate enhance the performance of carob seedlings grown...
Gama, Florinda; Correia, Pedro; Saavedra, Teresa; Dandlen, Susana; de Varennes, Amarilis; Nolasco, Gustavo; Pestana, MaribelaCorrection to: Journal of Soil Science and Plant Nutrition https://doi.org/10.1007/s42729-019-00100-4
Olive mild mosaic virus coat protein and p6 are suppressors of RNA silencing an...
Varanda, Carla M. R.; Materatski, Patrick; Campos, Maria Doroteia; Clara, Maria Ivone E.; Nolasco, Gustavo; Felix, Maria do RosarioRNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this mechanism. In this study, the genome of the Olive mild mosaic virus (OMMV) was screened for silencing suppressors. The full OMMV cDNA and 5 OMMV open reading frames (ORFs) were cloned into the Gateway binary vector pK7WG2, transformed into Agrobacterium tumefaciens, and agroinfiltrated into N....