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Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol ...

Charão,Mariele F.; Moro,Angela M.; Brucker,Natália; Bulcão,Rachel P.; Baierle,Marília; Freitas,Fernando; Durgante,Juliano; Nascimento,Sabrina

A method for the simultaneous quantification of lycopene, β-carotene, retinol and α-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20°C for...

Date: 2012 | Origin: Oasisbr

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