6 documents found, page 1 of 1
Análise de diferentes primers utilizados na PCR visando ao diagnóstico da tuber...
Ogusku, Maurício Morishi; Salem, Júlia Ignez
Evaluation of polymerase chain reaction in the diagnosis of pulmonary tuberculo...
Santos, Maria Cristina dos; Ogusku, Maurício Morishi; Miranda, José de Moraes; Santos, Maria Cristina dos; Salem, Júlia IgnezObjective: To evaluate the accuracy of bacteriological methods and of polymerase chain reaction (with primers specific for IS6110 of the Mycobacterium tuberculosis complex) in testing sputum samples from indigenous (Amerindian) and non-indigenous patients. Methods: A total of 214 sputum samples (154 from indigenous patients and 60 from nonindigenous patients) were analyzed in order to determine the accuracy of ...
PCR in the diagnosis of cutaneous tuberculosis
Ogusku, Maurício Morishi; Sadahiro, Aya; Hirata, Mario Hiroyuki; Hirata, Rosario Domínguez Crespo; Zaitz, Clarisse; Salem, Júlia IgnezSeeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated (with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH) biopsies tissues samples stored at -20°C. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% ...
Effects of culture filtrates of endophytic fungi obtained from Piper aduncum L....
Lima, Alita Moura de; Salem, Júlia Ignez; Souza, João Vicente Braga de; Cortêz, Ana Cláudia Alves; Carvalho, Clarice Maia; Chaves, Francisco Célio MaiaSubstances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following me...
Avaliação do protocolo PCR4 de Marchetti em tecidos parafinizados para o diagnó...
Fujimoto, Luciana Botinelly Mendonça; Salem, Júlia Ignez; Ogusku, Maurício Morishi; Ferreira, Luiz Carlos de Lima
Educational intervention for collecting sputum for tuberculosis: A quasi-experi...
Sicsú, Amélia Nunes; Salem, Júlia Ignez; Fujimoto, Luciana Botinelly Mendonça; Gonzáles, Roxana Isabel Cardozo; Cardoso, Maria do Socorro de LucenaObjective: to evaluate the quality of the sputum sample before and after the Nursing guidance to patients. Methods: this is a quasi-experimental research design, single group type, before and after, non-randomized study. The study enrolled patients with suspected pulmonary tuberculosis, respiratory symptomatic patients for over three weeks, aged over 18 years, of both genders and without tuberculosis history in...