78 documents found, page 1 of 8
Paper-based biomimetic test-strip for CA15-3 with coloured readout
Carneiro, Mariana; Rodrigues, L. R.; Moreira, Felismina; Sales, M. G. F.The combination of molecularly-imprinted polymers (MIPs) on paper substrates for capturing a cancer antigen 153 (CA15-3) with the traditional coloured transduction scheme of enzyme-linked immunosorbent assay (ELISA) assays using 3,3,5,5-tetramethylbenzidine (TMB), horseradish peroxidase (HRP) and H2O2 is presented here for the first time. Here, the paper surface was modified with a silane derivative containing ...
Aptasensor for the detection of Moraxella catarrhalis adhesin UspA2
Sande, Maria; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Melo, Luís Daniel Rodrigues; Saragliadis, Athanasios; Linke, DirkInnovative point-of-care (PoC) diagnostic platforms are desirable to surpass the deficiencies of conventional laboratory diagnostic methods for bacterial infections and to tackle the growing antimicrobial resistance crisis. In this study, a workflow was implemented, comprising the identification of new aptamers with high affinity for the ubiquitous surface protein A2 (UspA2) of the bacterial pathogen Moraxella ...
Fuel cells operating as an immunosensor for cancer biomarker screening
Ferreira, Nádia; Carneiro, Liliana; Pinto, Alexandra M. F. R.; Sales, M. G. F.This work presents the first integration of antibodies into one of the electrodes of a passive direct methanol fuel cell (DMFC) to create an autonomous immunosensor for cancer antigen 153 (CA15-3). The anode of the fuel cell was first made of carbon cloth with carbon black, platinum and ruthenium nanoparticles and then modified with anti-CA15-3, while the cathode was made of carbon cloth with carbon black and p...
Development of an innovative flexible paper-based methanol fuel cell (PB-DMFC) ...
Carneiro, Liliana; Pinto, Alexandra M. F. R.; Sales, M. G. F.This work describes for the first time a paper-based direct methanol fuel cell platform (PB-DMFC) that functions as an energy source and biosensor, assembled on a simple paper substrate for point-of-care (POC) applications, targeting sarcosine as proof-of-concept. Specifically, a methanol fuel cell strip was developed from a square of Whatman paper, acting as substrate. The paper strip was treated with an imper...
Flexible sensing devices integrating molecularly-imprinted polymers for the det...
Martins, G. V.; Riveiro, A.; Chiussi, S.; Sales, M. G. F.In recent years, the development of flexible and wearable devices for healthcare and biomedical applications has become an emerging technological goal, particularly with personalized medicine on the rise. As a response to the increasing demand for in-situ sensing platforms that fulfil some essential requirements like sensitivity, reproducibility and high stability, electrochemical sensors have boosted their way...
Wine astringent compounds monitored by an electrochemical biosensor
Costa, Joana J.; Moreira, Felismina; Soares, Susana; Brandão, Elsa; Mateus, Nuno; Freitas, Victor De; Sales, M. G. F.An innovative approach for monitoring astringent polyphenols in beverages (wines) is described, consisting of an electrochemical biosensor constructed by adsorbing salivary -amylase or proline-rich protein (PRP) onto amined gold screen-printed electrodes. Interaction with polyphenols was tested using pentagalloyl glucose (PGG) as a standard, an important representative element for astringency. The analytical pr...
Flexible sensing devices integrating molecularly-imprinted polymers for the det...
Martins, Gabriela Ferreira Vasconcelos; Riveiro, A.; Chiussi, S.; Sales, M. G. F.In recent years, the development of flexible and wearable devices for healthcare and biomedical applications has become an emerging technological goal, particularly with personalized medicine on the rise. As a response to the increasing demand for in-situ sensing platforms that fulfil some essential requirements like sensitivity, reproducibility and high stability, electrochemical sensors have boosted their way...
Immunosensor for alpha-synuclein detection in Parkinson´s Disease
Silva, Inês; Martinho, Vera; Cardoso, Rita; Sales, M. G. F.Parkinsons disease (PD) is a neurodegenerative disorder and one of the leading causes of death worldwide [1]. The principal neuropathological defining hallmark of PD is the presence of Lewy bodies mainly composed of aggregates of alpha-synuclein (-syn) [2]. This protein is a specific PD biomarker that, when detected early, can contribute to an effective PD treatment application. Immunosensors are analytical pla...
Paper-based aptasensor for colorimetric detection of osteopontin
Pereira, Ana Cláudia; Moreira, Felismina; Rodrigues, L. R.; Sales, M. G. F.This work presents a novel cellulose-based aptasensor for the colorimetric detection of a cancer biomarker, osteopontin (OPN), in point-of-care (PoC) analysis. For this purpose, the cellulose paper was chemically modified with (mercaptopropyl)methyldimetoxisilane to attach the thiolated aptamer, which acts as a biological detection layer. The surface modification was checked by Fourier transform infrared spectr...
Gold nanoparticles as therapeutic agent for radiotherapy of PC3 prostate cancer...
Soares, Sílvia; Guerreiro, Susana; Sales, M. G. F.; Correa-Duarte, Miguel; Fernandes, RúbenRadiotherapy (RT) is a therapeutic modality that deliver a precise dose of ionizing radiation in a specific tumour volume, promoting the irradiation of tumor cells with as minimal damage as possible in surrounding normal tissues. Besides the evolution of equipment and technology, this therapy still has some problems related with radioresistance, which consequently increase the recurrence at 5 years. Over the ye...