121 documents found, page 1 of 13
Amplified Sensitivity in SERS Detection of L1CAM With Silver Plasmonic Mesoporo...
Castaño-Guerrero, Yuselis; Arjones-Fernández, Belén; Moreira, Felismina T. C.; Alvarez-Puebla, Ramon A.; Correa-Duarte, Miguel A.; Águas, H.This study presents a novel approach for dual detection, leveraging a combination of a Raman reporter-bearing nanomaterial and molecular imprinting polymers (MIP). A core-shell Au-Ag nanoparticles (Au-Ag NPs) encapsulated in mesoporous silica nanocapsules (Au-Ag NCs) and a new MIP-based material targeting L1CAM are used. The MIP prepared via surface imprinting on a carbon screen-printed electrode (C-SPE) used t...
Paper-based biomimetic test-strip for CA15-3 with coloured readout
Carneiro, Mariana C. C. G.; Rodrigues, Lígia R.; Moreira, Felismina; Sales, M. Goreti F.The combination of molecularly-imprinted polymers (MIPs) on paper substrates for capturing a cancer antigen 15–3 (CA15-3) with the traditional coloured transduction scheme of enzyme-linked immunosorbent assay (ELISA) using 3,3′,5,5′-tetramethylbenzidine (TMB), horseradish peroxidase (HRP) and H2O2 is presented here for the first time. Here, the paper surface was modified with a silane derivative containing an a...
CuMV VLPs Containing the RBM from SARS-CoV-2 Spike Protein Drive Dendritic Cell...
Sebastião, Ana Isabel; Mateus, Daniela; Carrascal, Mylène A.; Sousa, Cátia; Cortes, Luísa; Bachmann, Martin F; do Carmo, Anália; Matos, Ana M.Dendritic cells (DCs) are the most specialized and proficient antigen-presenting cells. They bridge innate and adaptive immunity and display a powerful capacity to prime antigen-specific T cells. The interaction of DCs with the receptor-binding domain of the spike (S) protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a pivotal step to induce effective immunity against the S protei...
Metabolic Disruption of Gold Nanospheres, Nanostars and Nanorods in Human Metas...
Soares, Sílvia; Pereira, Cláudia; Sousa, André P; Oliveira, Ana Catarina; Sales, M. Goreti F.; Correa-Duarte, Miguel A; Guerreiro, Susana GNanomaterials offer a broad spectrum of applications in biomedicine. The shapes of gold nanoparticles could modulate tumor cell behavior. Spherical (AuNPsp), stars (AuNPst) and rods (AuNPr) shapes of polyethylene glycol coated-gold nanoparticles (AuNPs-PEG) were synthesized. Metabolic activity, cellular proliferation, and reactive oxygen species (ROS) were measured and the impact of AuNPs-PEG in metabolic enzym...
Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Ca...
Soares, Sílvia; Faria, Isabel; Aires, Fátima; Monteiro, Armanda; Pinto, Gabriela; Sales, M. Goreti F.; Correa-Duarte, Miguel A.; Guerreiro, Susana G.More than 50% of all prostate cancer (PCa) patients are treated by radiotherapy (RT). Radioresistance and cancer recurrence are two consequences of the therapy and are related to dose heterogeneity and non-selectivity between normal and tumoral cells. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome these therapeutic limitations of RT. This study assessed the biological interac...
Development of an innovative flexible paper-based methanol fuel cell (PB-DMFC) ...
Carneiro, Liliana P.T.; Pinto, Alexandra M.F.R.; Sales, M. Goreti F.This work describes for the first time a paper-based direct methanol fuel cell platform (PB-DMFC) that functions as an energy source and biosensor, assembled on a simple paper substrate for point-of-care (POC) applications, targeting sarcosine as proof-of-concept. Specifically, a methanol fuel cell strip was developed from a square of Whatman paper, acting as substrate. The paper strip was treated with an imper...
Bacterial nanocellulose membrane as novel substrate for biomimetic structural c...
Suleimenova, Akmaral; Frasco, Manuela F.; Soares da Silva, Francisco A.G.; Gama, Miguel; Fortunato, Elvira; Sales, M. Goreti F.The development of optical biosensors based on structural colors generated by short-range ordered colloidal particles is attracting growing interest due to their non-iridescent and non-fading features. In this study, a biomimetic approach using biopolymers for the various steps of sensor construction is presented. Bacterial nanocellulose (BNC) has many foreseen applications in biomedical engineering because of ...
Detection of serum calprotectin based on molecularly imprinted photonic hydroge...
Resende, Sara; Frasco, Manuela F.; Freitas, Paulo P.; Sales, M. Goreti F.Inflammatory bowel disease (IBD) affects millions of people worldwide, and current diagnosis relies on a number of complex procedures. The need for sensitive diagnostic tools has focused research on discovering new biomarkers and improving detection methods. Serum calprotectin has recently emerged as a new serological biomarker and shows great potential due to its high specificity. In this work, a label-free bi...
Fuel cells operating as an immunosensor for cancer biomarker screening
Ferreira, Nádia; Carneiro, Liliana; Pinto, Alexandra M. F. R.; Sales, M. Goreti F.This work presents the first integration of antibodies into one of the electrodes of a passive direct methanol fuel cell (DMFC) to create an autonomous immunosensor for cancer antigen 15–3 (CA15-3). The anode of the fuel cell was first made of carbon cloth with carbon black, platinum and ruthenium nanoparticles and then modified with anti-CA15-3, while the cathode was made of carbon cloth with carbon black and ...
A cork based substrate coupled with artificial antibodies for point-of-care det...
Correia, Bárbara; Oliveira, Daniela; Vulpe, Georgeta; Tavares, Ana P. M.; Sales, M. Goreti F.; Duarte, Abel J.; Sharma, Sanjiv; Moreira, Felismina T. C.Whilst single use point of care testing (PoCT) devices have transformed healthcare globally, there are major concerns over their environmental consequences. These concerns could be addressed by employing devices made of environmentally friendly materials. Herein, we report on the use of cork based PoCT devices. Cork is known to be fully biodegradable and can be easily recycled without producing toxic residues. ...