11 documents found, page 1 of 2
SIGAA: signaling automated analysis: a new tool for Ca2+ signaling quantificati...
Lopes, Rafael Faria; Gonçalves-Ribeiro, Joana; Sebastião, Ana M.; Meneses, Carlos; Vaz, Sandra H.Astrocytes are non-neural cells, restricted to the brain and spinal cord, whose functions and morphology depend on their location. Astrocyte–astrocyte and astrocyte–neuron interactions occur through cytoplasmic Ca2+ level changes that are assessed to determine cell function and response (i.e., drug testing). The evaluation of alterations in intracellular Ca2+ levels primarily relies on fluorescence imaging tech...
Changes in adenosine receptors and neurotrophic factors in the SOD1G93A mouse m...
Rei, Nádia; Valente, Cláudia A.; Vaz, Sandra H.; Farinha-Ferreira, Miguel; Ribeiro, Joaquim A.; Sebastião, Ana M.Amyotrophic lateral sclerosis (ALS) is characterized by the progressive degeneration of corticospinal tract motor neurons. Previous studies showed that adenosine-mediated neuromodulation is disturbed in ALS and that vascular endothelial growth factor (VEGF) has a neuroprotective function in ALS mouse models. We evaluated how adenosine (A1R and A2AR) and VEGF (VEGFA, VEGFB, VEGFR-1 and VEGFR-2) system markers ar...
The neurosphere assay : an effective in vitro technique to study neural stem cells
Xapelli, Sara; Soares, Rita; Ribeiro, Filipa; Lourenço, Diogo M.; Rodrigues, Rui S.; Moreira, João B.; Sebastião, Ana M.; Morais, Vanessa A.Neural stem cells (NSCs) are known to be present in the adult mammalian brain where they constitutively differentiate into the neuronal, astroglial, and oligodendroglial lineages, in defined processes termed n e u ro ge n e s i s , a st ro g l i o ge n e s i s a n d oligodendrogenesis, respectively (reviewed in Braun and Jessberger, 2014). During brain development, NSCs are present throughout the brain, becomin...
Microglia Dysfunction Caused by the Loss of Rhoa Disrupts Neuronal Physiology a...
Socodato, Renato; Portugal, Camila C; Canedo, Teresa; Rodrigues, Artur Filipe; Almeida, Tiago O; Henriques, Joana F; Vaz, Sandra H; Magalhães, JoãoNervous tissue homeostasis requires the regulation of microglia activity. Using conditional gene targeting in mice, we demonstrate that genetic ablation of the small GTPase Rhoa in adult microglia is sufficient to trigger spontaneous microglia activation, producing a neurological phenotype (including synapse and neuron loss, impairment of long-term potentiation [LTP], formation of β-amyloid plaques, and memory ...
Microglia dysfunction caused by the loss of rhoa disrupts neuronal physiology a...
Socodato, Renato; Portugal, Camila C.; Canedo, Teresa; Rodrigues, Artur; Almeida, Tiago O.; Henriques, Joana F.; Vaz, Sandra H.; Magalhães, JoãoNervous tissue homeostasis requires the regulation of microglia activity. Using conditional gene targeting in mice, we demonstrate that genetic ablation of the small GTPase Rhoa in adult microglia is sufficient to trigger spontaneous microglia activation, producing a neurological phenotype (including synapse and neuron loss, impairment of long-term potentiation [LTP], formation of β-amyloid plaques, and memory ...
Modulation of subventricular zone oligodendrogenesis: a role for hemopressin?
Xapelli, Sara Alves; Agasse, Fabienne; Grade, Sofia; Bernardino, Liliana; Ribeiro, Filipa F.; Schitine, Clarissa S.; Heimann, Andrea S.; Ferro, Emer S.Neural stem cells (NSCs) from the subventricular zone (SVZ) have been indicated as a source of new oligodendrocytes to use in regenerative medicine for myelin pathologies. Indeed, NSCs are multipotent cells that can self-renew and differentiate into all neural cell types of the central nervous system. In normal conditions, SVZ cells are poorly oligodendrogenic, nevertheless their oligodendrogenic potential is b...
Activation of type 1 cannabinoid receptor (CB1R) promotes neurogenesis in murin...
Xapelli, Sara Alves; Agasse, Fabienne; Sardà-Arroyo, Laura; Bernardino, Liliana; Santos, Tiago; Ribeiro, Filipa F.; Valero, Jorge; Bragança, JoséThe endocannabinoid system has been implicated in the modulation of adult neurogenesis. Here, we describe the effect of type 1 cannabinoid receptor (CB1R) activation on self-renewal, proliferation and neuronal differentiation in mouse neonatal subventricular zone (SVZ) stem/progenitor cell cultures. Expression of CB1R was detected in SVZ-derived immature cells (Nestin-positive), neurons and astrocytes. Stimulat...
Extracellular Alpha-Synuclein Oligomers Modulate Synaptic Transmission and Impa...
Diógenes, Maria José; Dias, Raquel B.; Rombo, Diogo M.; Miranda, Hugo Vicente; Maiolino, Francesca; Guerreiro, Patrícia; Näsström, ThomasParkinson’s disease (PD) is the most common representative of a group of disorders known as synucleinopathies, in which misfolding and aggregation of -synuclein (a-syn) in various brain regions is themajorpathological hallmark. Indeed, themotorsymptomsinPDare causedby a heterogeneous degeneration of brain neurons not only in substantia nigra pars compacta but also in other extrastriatal areas of the brain. In a...
Adenosine A2A receptors control the extracellular levels of adenosine through m...
Pinto-Duarte, António; Coelho, Joana E.; Cunha, Rodrigo A.; Ribeiro, Joaquim Alexandre; Sebastião, Ana M.Adenosine, a neuromodulator of the CNS, activates inhibitory-A1 receptors and facilitatory-A2A receptors; its synaptic levels are controlled by the activity of bi-directional equilibrative nucleoside transporters. To study the relationship between the extracellular formation/inactivation of adenosine and the activation of adenosine receptors, we investigated how A1 and A2A receptor activation modifies adenosine...
Endogenous Adenosine Modulation of 22Na Uptake by Rat Brain Synaptosomes
Ribeiro, Joaquim Alexandre; Lobo, M. Graça B.; Sebastião, Ana M.To evaluate if endogenous extracellular adenosine influences sodium channel activity in nerve terminals, we investigated how manipulations of extracellular adenosine levels influence 22Na uptake by rat brain synaptosomes stimulated with veratridine (VT). To decrease extracellular adenosine levels, adenosine deaminase (ADA) that converts adenosine into an inactive metabolite was used. To increase extracellular a...