Replacing traditional agricultural ingredients with biotechnologically improved agro-industry by-products in fish diets promotes sustainable aquaculture, reduces production costs and carbon footprint, and promotes a circular economy. Brewers spent grain (BSG) is one such by-product. Solid-state fermentation (SSF) of BSG with Aspergillus ibericus enhances its nutritional value and digestibility for European seab...
The growing global demand for protein-rich foods requires sustainable alternatives to the conventional animal based protein systems. In this sense, insect-based proteins, especially from Tenebrio molitor, present a promising nutrient-rich solution with a reduced ecological footprint. Nonetheless, insect meal is composed of chitin and complex proteins, limiting their digestibility. This study aimed to enhance th...
Solid state fermentation is a biotechnological process that allows the utilization of agro-industrial by-products as solid substrates for the growth of microorganisms, including filamentous fungi and yeasts, with the simultaneous production of added value compounds, like enzymes. Oil cakes, such as olive and sunflower cakes, are generated during vegetable oil extraction and have received great interest as solid...
Lignocellulosic biomass is a promising feedstock for added value compound production in biotechnological processes such as solid-state fermentation (SSF). Although these solid materials can be directly used as substrates in fermentations in a solid state, a pretreatment is often required, especially if the microorganism selected is unable to produce lignocellulosic enzymes. In the present work, several pretreat...
Oilseed cakes (OC) present high potential as feedstock for the biobased industry. Biotechnological processes allow OC valorization by the production of diverse value-added products and simultaneously altering OC structure, improving their nutritional value, and boosting OC utilization in animal feed. This work explored the use of fungi consortium of two different species as a bioprocessing approach to improve t...
Fortifying fish feeds with bioactive compounds, such as enzymes and antioxidants, has been an adopted strategy to improve feed nutritional quality and sustainability. However, feed additives can lose activity/effectiveness during pelleting and storage processes. This work aimed to monitor functional activity stability in feeds supplemented with a bioactive extract, including cellulases, xylanases, and antioxida...
Industrial agar extraction of red seaweed Gelidium produces a significant amount of by- products (red seaweed by-product - RSB), which are often discarded. Nevertheless, their rich composition in carbohydrates and proteins makes them appropriate for value-added compound production through solid-state fermentation (SSF). In this study, RSB was utilized as substrate for SSF with Aspergillus ibericus MUM 03.49 and...
Sunflower (SFC), rapeseed (RSC), and soybean cakes (SBC) are examples of oilseed cakes (OC) used in animal feed. Bioprocessing of these OC by solid-state fermentation (SSF) aims to boost OC applications in feed and other industries by reducing antinutritional factors and releasing enzymes and antioxidants. A simplex centroid design was performed to optimize the substrate composition of SFC, RSC and SBC mixtures...
Vegetable oil extraction generates high amounts of by-products, which are designated as oil cakes. Since the current strategies employed for oil cakes reuse are linked with some drawbacks, identification of alternative approaches to decrease the environmental impact and promote a circular economy is of vital importance. In general, these materials are characterized by high fiber content, making them suitable to...
Vegetable oil industries are very relevant in the Portuguese agro-food sector, with around 350 thousand tonnes of vegetable oils produced in 2017 (INE, 2018). The extraction of these oils from seeds or fruits is associated with the production of high amounts of solid by-products, named oil cakes, and the development of new and eco-friendly strategies for their reuse and valorization is essential (Lomascolo et a...