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Growth, oxidative stress, and intermediary metabolism of European seabass (Dice...

Filipe, Diogo; Magalhães, Rui; Fernandes, Helena; Salgado, José Manuel Seara; Belo, Isabel; Oliva-Teles, Aires; Peres, Helena

Previous research demonstrated that solid-state fermentation (SSF) of distiller's dried grains with solubles (DDGS) by Aspergillus ibericus improved protein, reduced neutral and acid detergent fiber levels, and added phenolic compounds, xylanase, and cellulase. Fermentation of DDGS also increased protein, lipids, energy, and starch digestibility. This study evaluated the effects of unfermented and fermented DDG...


Effects of solid-state fermentation of Gelidium corneum by-product on immune st...

Ferreira, Marta Alexandra Lages; Ramos-Oliveira, Catarina; Magalhães, Rui; Martins, Nicole; Serra, Cláudia R.; Salgado, José Manuel Seara; Belo, Isabel

It was previously observed that dietary inclusion of 10 % Gelidium corneum by-product and sunflower cake mixture (1:1 ratio; Gmix), replacing corn gluten meal, reduced feed utilization efficiency in European sea bass juveniles, while the same mixture fermented with Aspergillus ibericus restored feed efficiency as well as nitrogen and energy utilization, achieving levels comparable to the control diet. The prese...


Aspergillus ibericus biomass production by submerged fermentation of Codium tom...

Ferreira, Marta Alexandra Lages; Salgado, José Manuel Seara; Peres, Helena; Belo, Isabel

Seaweeds are potential feedstocks for biorefinery approaches, owing to their carbohydrate content, high biomass production and lack of lignin content, which makes saccharification more feasible. The seaweed sugar-rich hydrolysates can be used to produce alternative protein sources, such as mycoprotein, to meet the increasing global protein needs in a sustainable way. Therefore, this study aimed to produce bioma...


Fermented agar by-product and sunflower cake mixture as feedstuff for European ...

Ferreira, Marta; Ramos-Oliveira, Catarina; Magalhães, Rui; Martins, Nicole; Ozório, Rodrigo O. A.; Salgado, José Manuel Seara; Belo, Isabel

Industrial agar production from red seaweeds such as Gelidium sp. generates large quantities of byproducts (GBP), often discarded as waste. GBP and sunflower cake (SC) are under-valorized as feedstuff due to their high non-starch polysaccharide (NSP) content. Solid-state fermentation (SSF) may disrupt NSP and improve their nutritional value. This study evaluated the utilization of Aspergillus ibericus and Asper...


Potential of solid-state fermentation to enhance the nutritional value of oilse...

Sousa, Daniel; Moset, Verónica; López-Luján, María del Carmen; Salgado, José Manuel Seara; Dias, Alberto Carlos Pires; Belo, Isabel; Pascual, Juan José

The objective of this study was to evaluate the potential of solid-state fermentation (SSF) to enhance the nutritional value of oilseed cakes (OC) for poultry. To this end, firstly, we characterized the main nutrients and antinutritional factors in non-fermented OC and fermented OC, as well as in fermented and extracted OC, from where enzymatic extracts were recovered. The fermented and extracted OC represented...


Valorization of Gelidium corneum by-product through solid-state fermentation

Ferreira, Marta Alexandra Lages; Salgado, José Manuel Seara; Peres, Helena; Belo, Isabel

Red seaweed Gelidium is used for agar extraction, generating large quantities of by-products (red seaweed by-product RSB). Its carbohydrate and protein-rich composition makes it a suitable substrate to produce value-added compounds through solid-state fermentation (SSF). In this study, RSB was used in SSF with Aspergillus ibericus and Aspergillus niger for carbohydrases production and protein enrichment. RSB wa...


Bioprocessamento de produtos derivados de insetos pela leveduraYarrowia lipolytica

Dias, Bruna Filipa Silva; Araújo, Liliana Patrícia Silva; Araújo, Joana Isabel; Salgado, José Manuel Seara; Venâncio, Armando; Lopes, Marlene

Atualmente, a procura por novas fontes alimentares está a crescer de forma significativa, com o objetivo de responder à expectável e crescente necessidade de alimentos nos próximos anos. Nesse sentido, a FAO (Organização das Nações Unidas para Alimentação e Agricultura) e a União Europeia têm promovido a incorporação de insetos como uma alternativa de proteína sustentável e altamente nutritiva para o consumo hu...


Evaluation of biotechnological processing through solid-state fermentation of o...

Sousa, Daniel; Simões, Luara Aparecida; Oliveira, Rui Pedro Soares de; Salgado, José Manuel Seara; Cambra-López, Maria; Belo, Isabel

Oilseed cakes (OC) are natural sources of lignocellulosic biomass, produced every year in large amounts. In addition to their main applications as animal feed, plant or soil fertilizer, and compost, they present enormous potential for being used in biotechnological processes for the obtainment and extraction of valuable bioactive compounds. This work evaluated the effect of solid-state fermentation on the bioac...


Pre-treatment of Ulva rigida improves its nutritional value for European seabas...

Fernandes, Helena; Martins, Nicole; Vieira, Lúcia; Salgado, José Manuel Seara; Castro, Carolina; Oliva-Teles, Aires; Belo, Isabel; Peres, Helena

Macroalgae are promising ingredients for aquafeeds, but their recalcitrant polysaccharide structure limits their wide use. To disrupt this structure, different physical (ultra-sounds, autoclave, microwaves), chemical (acid, alkaline), and biotechnological (solid-state fermentation, SSF; SSF followed by sequential hydrolysis, SSF-SH) treatments were carried out in Ulva rigida. The chemical composition, morpholog...


Enzyme-rich extract and use thereof in pre-treatment of plant feedstuff-based d...

Peres, Helena; Belo, Isabel; Salgado, José Manuel Seara; Castro, Carolina; Teles, Aires Oliva; Fernandes, Helena; Moyano, Francisco J.

The present disclosure relates to an enzyme-rich extract obtained through solid-state fermentation (SSF) of Brewer's Spent Grain (BSG) to be used as a feed additive in animal diets, preferably fish diets. The present patent application also discloses a pre-treatment process of plant feedstuff-based (PF) diets in order to increase the nutrient bioavailability of said diets, thus increasing their nutritional value.


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