4 documents found, page 1 of 1
A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substit...
Salgado, Rafael Locatelli; Vidigal, Pedro Marcus Pereira; Gonzaga, Natalia F.; Souza, Luiz F. L. de; Polêto, Marcelo D.; Onofre, Thiago SouzaPorcine circovirus-2 (PCV2) is the etiologic agent of several diseases in pigs, including multi-systemic wasting syndrome (PMWS). In this work, a new mutant PCV2b was isolated from PMWS-affected pigs on a Brazilian farm. Its genome showed high sequence similarity (>99 % identity) to those from a group of emerging mutants isolated from cases of PMWS outbreaks in vaccinated pigs in China, the USA and South Korea....
Ser2 from Serratia liquefaciens L53: A new heat stable protease able to destabi...
Baglinière, François; Tanguy, Gaëlle; Salgado, Rafael Locatelli; Jardin, Julien; Rousseau, Florence; Robert, Benoît; Harel-Oger, MarielleThe heat-stable protease Ser2 is secreted by the species Serratia liquefaciens, a psychrotrophic bacteria frequently found in raw milk. To understand the physicochemical modifications of casein micelles induced by Ser2 and to confirm its implication in UHT milk destabilization, the enzyme was purified and added to microfiltered raw milk before UHT treatment. UHT milk destabilization was investigated during 90 d...
Utilization of phage display to identify antigenic regions in the PCV2 capsid p...
Santos, Marcus Rebouças; Assao, Viviane Sisdelli; Salgado, Rafael Locatelli; Fietto, Juliana Lopes Rangel; Bressan, Gustavo CostaPorcine circovirus 2 (PCV2) is associated with a series of swine diseases. There is a great interest in improving our understanding of the immunology of PCV2, especially the properties of the viral capsid protein Cap-PCV2 and how they relate to the immunogenicity of the virus and the subsequent development of vaccines. Phage display screening has been widely used to study binding affinities for target proteins....
Potential antileukemia effect and structural analyses of SRPK inhibition by N-(...
Siqueira, Raoni Pais; Barbosa, Éverton de Almeida Alves; Polêto, Marcelo Depólo; Righetto, Germanna Lima; Seraphim, Thiago VargasDysregulation of pre-mRNA splicing machinery activity has been related to the biogenesis of several diseases. The serine/arginine-rich protein kinase family (SRPKs) plays a critical role in regulating pre-mRNA splicing events through the extensive phosphorylation of splicing factors from the family of serine/arginine-rich proteins (SR proteins). Previous investigations have described the overexpression of SRPK1...