5 documents found, page 1 of 1
Identification and functional analysis of novel genes expressed in the Anterior...
Gonçalves Dias Da Silva, Lisa; Filipe, Mario; Marques, Sara; Salgueiro, Ana Marisa; Becker, Jorg D.; Belo, José A.During early vertebrate development, the correct establishment of the body axes is critical. The anterior pole of the mouse embryo is established when Distal Visceral Endoderm (DVE) cells migrate to form the Anterior Visceral Endoderm (AVE). Symmetrical expression of Lefty1, Cer1 and Dkk1 determines the direction of DVE migration and the future anterior side. In addition to the establishment of the Anterior-Pos...
Functional study of genes expressed in the AVE during mouse embryonic developme...
Salgueiro, Ana MarisaA presente dissertação insere-se no âmbito do estudo do desenvolvimento embrionário, uma área de crescente interesse que integra biologia molecular e celular, genética, fisiologia, neurobiologia e imunologia. O modelo animal escolhido para estes estudos foi o ratinho (mus musculus). Entre Humano e ratinho existe uma conservação evolucionária que se reflecte ao nível do genoma, partilhando entre eles 99% da sequ...
A secreted BMP antagonist, Cer1, fine tunes the spatial organization of the ure...
Chi, Lijun; Saarela, Ulla; Railo, Antti; Prunskaite-Hyyrylainen, Renata; Skovorodkin, Ilya; Anthony, Shelagh; Katsu, Kenjiro; Liu, Yu; Shan, JingdongThe epithelial ureteric bud is critical for mammalian kidney development as it generates the ureter and the collecting duct system that induces nephrogenesis in dicrete locations in the kidney mesenchyme during its emergence. We show that a secreted Bmp antagonist Cerberus homologue (Cer1) fine tunes the organization of the ureteric tree during organogenesis in the mouse embryo. Both enhanced ureteric expressio...
Generating asymmetries in the early vertebrate embryo: the role of the Cerberus...
Belo, José A.; Silva, Ana C.; Borges, Ana; Filipe, Mário; Bento, Margaret; Gonçalves, Lisa; Vitorino, Marta; Salgueiro, Ana Marisa; Texeira, VeraOne fundamental aspect of vertebrate embryonic development is the formation of the body plan. For this process, asymmetries have to be generated during early stages of development along the three main body axes: Anterior-Posterior, Dorso-Ventral and Left-Right. We have been studying the role of a novel class of molecules,the Cerberus/Dan gene family. These are dedicated secreted antagonists of three major signa...
N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase expression during early mo...
Salgueiro, Ana Marisa; Filipe, Mario; Belo, José A.N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST) is an enzyme which is known to help build up the GlcA beta 1-3GalNAc(4,6-bisSO(4)) unit of chondroitin sulfate E (CSE). This enzymatic activity has been reported in squid cartilage and in human serum, but has never been reported as an enzyme required during early mouse development. On the other hand, CSE has been shown to bind with strong affin...