3 documents found, page 1 of 1
An EXAFS Approach to the Study of Polyoxometalate–Protein Interactions: The Cas...
Marques, M. Paula M.; Gianolio, Diego; Ramos, Susana; Carvalho, Luís A. E. Batista de; Aureliano, ManuelEXAFS and XANES experiments were used to assess decavanadate interplay with actin, in both the globular and polymerized forms, under different conditions of pH, temperature, ionic strength, and presence of ATP. This approach allowed us to simultaneously probe, for the first time, all vanadium species present in the system. It was established that decavanadate interacts with G-actin, triggering a protein conform...
An EXAFS approach to the study of polyoxometalate–proteiniInteractions: the cas...
Marques, M. P. M.; Gianolio, Diego; Ramos, Susana; Carvalho, Luís E. A. B.; Aureliano, M.Decavanadate−actin interactions were studied by EXAFS and XANES, allowing us to simultaneously probe, for the first time, all vanadium species in the system. V10 interacts with G-actin (at its ATP binding site), a V−SCys coordination having been verified along with oxidovanadium formation. V10’s interplay with F-actin was found to be weak and occur via a different mechanism. These results are relevant for under...
A molecular view of cisplatin's mode of action: interplay with DNA bases and ac...
Marques, M. P. M.; Gianolio, Diego; Cibin, Giannantonio; Tomkinson, John; Parker, Stewart F.; Valero, Rosendo; Pedro Lopes, RThe interaction of the widely used anticancer drug cisplatin with DNA bases was studied by EXAFS and vibrational spectroscopy (FTIR, Raman and INS), coupled with DFT/plane-wave calculations. Detailed information was obtained on the local atomic structure around the Pt(ii) centre, both in the cisplatin-purine (adenine and guanine) and cisplatin-glutathione adducts. Simultaneous neutron and Raman scattering exper...