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Elemental biochemical analysis of the polysaccharides in the extracellular matr...

Oliveira, Fábio Luís Silva Faria; Carvalho, Joan; Belmiro, Celso L. R.; Ramalho, Gustavo; Pavão, Mauro; Lucas, Cândida; Ferreira, Célia

In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown. Saccharomyces cerevisiae ECM was produced, extracted and partitioned. An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography–mass ...


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