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Elderberry Stalks as a Source of High-Value Phytochemical: Essential Minerals a...

Patinha, Samuel; Murteira, Juliana V.; Costa, Carina Pedrosa; Salvador, Ângelo C.; Santos, Sónia A.O.; Silvestre, A.J.D.; Rocha, Sílvia M.

Elderberry (Sambucus nigra L.) consumption has been growing in the last years, generating a large number of stalks (~10% of the berries bunch) that are still under-valorized. This study focused on the evaluation of elderberry stalks as a source of high-value phytochemicals. In this vein, the essential mineral content and lipophilic composition were analyzed for the first time. In addition, the polar fraction wa...


Chemical characterization of Sambucus nigra L. flowers aqueous extract and its ...

Ferreira-Santos, P.; Costa, H.; Salvador, Ângelo C.; Silvestre, Armando J. D.; Santos, Sónia A. O.; Rocha, Sílvia M.; Sousa, Ana Margarida

The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 °C). The extract obtained at 90 °C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was analyzed by GC-MS and HPLC-MS, which allowed the identification ...


Strategies to preserve postharvest quality of horticultural crops and superfici...

Dias, Cindy; Amaro, A. L.; Salvador, Ângelo C.; Silvestre, Armando J.D.; Rocha, Sílvia M.; Isidoro, Nélson; Pintado, Manuela

Horticultural crops are vulnerable to several disorders, which affect their physiological and organoleptic quality. For about forty years, the control of physiological disorders (such as superficial scald) in horticultural crops, particularly in fruit, was achieved through the application of the antioxidant diphenylamine (DPA), usually combined with controlled atmosphere (CA) conditions. However, identification...


Antioxidant extracts from natural sources as potential enzymatic browning inhib...

Dias, Cindy; Amaro, Ana; Salvador, Ângelo C.; Rocha, Sílvia M.; Silvestre, Armando J. D.; Isidoro, Nélson; Pintado, Manuela


Three mammal species distinction through the analysis of scats chemical composi...

Saraiva, Maria J.; Salvador, Ângelo C.; Fernandes, Tony; Ferreira, Joaquim P.; Barros, António S.; Rocha, Sílvia M.; Fonseca, Carlos

In the last years, new approaches use scats analysis in ecological studies, like gas chromatographic techniques, has been developed. The aim of this study is to use VOCs (volatile organic compounds) released from scats to distinguish three mammal species: the ungulates red and fallow deer and the carnivore Eurasian otter through the application of SPME/GC??GC-ToFMS. This chromatographic technique has never been...


Can volatile organic metabolites be used to simultaneously assess microbial and...

Salvador, Ângelo C.; Baptista, Inês; Barros, António S.; Gomes, Newton C. M.; Cunha, Ângela; Almeida, Adelaide; Rocha, Silvia M.

A novel approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-ToFMS) was developed for the simultaneous screening of microbial and mite contamination level in cereals and coffee beans. The proposed approach emerges as a powerful tool for the rapid assessment of the microbial contamination level (ca...


Effect of the ionic liquid [bmim]Cl and high pressure on the activity of cellulase

Salvador, Ângelo C.; Santos, Mickael Da C.; Saraiva, Jorge A.

The effect of the ionic liquid 1-butyl-3-methylimidazolium chloride ([bmim]Cl) and of high pressure on the activity of cellulase from Aspergillus niger were studied separately and in combination. The enzyme activity decreased with increasing concentrations of [bmim]Cl, reaching 50% the value in aqueous buffer with 20% [bmim]Cl. However, when the enzyme is held in 10% [bmim]Cl and is then assayed in 1% [bmim]Cl,...


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