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Enhancement of methane production from 1-hexadecene by additional electron donors

1-Hexadecene-contaminated wastewater is produced in oil refineries and can be treated in methanogenic bioreactors, although generally at low conversion rates. In this study, a microbial culture able to degrade 1-hexadecene was enriched, and different stimulation strategies were tested for enhancing 1-hexadecene conversion to methane. Seven and three times faster methane production was obtained in cultures stimu...

Perspectives on carbon materials as powerful catalysts in continuous anaerobic ...

The catalytic effect of commercial microporous activated carbon (AC) and macroporous carbon nanotubes (CNT) is investigated in reductive bioreactions in continuous high rate anaerobic reactors, using the azo dye Acid Orange 10 (AO10) as model compound as electron acceptor and a mixture of VFA as electron donor. Size and concentration of carbon materials (CM) and hydraulic retention time (HRT) are assessed. CM i...

Effect of short chain fructooligosaccharides (scFOS) on immunological status an...

The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks. Overall, scFOS had only minor ...

Who is who in anaerobic oil biodegradation?

[Excerpt] Anaerobic bioremediation is an important alternative for the common aerobic cleanup of subsurface petroleum-contaminated soil and water. Microbial communities involved in anaerobic oil biodegradation are scarcely studied, and only few mechanisms of anaerobic hydrocarbons degradation are described. In this work, microbial degradation of aliphatic hydrocarbons (AHC) was studied by using culture-dependen...

Energy production from lipids by novel anaerobes

[Excerpt] Anaerobic microbial diversity encloses a very high potential that can be used for biotechnological applications. This potential is still largely unexplored, since the majority of the microorganisms in Nature are unknown or poorly characterized. This work is focused on the study of novel anaerobic microorganisms that participate in the metabolism of lipids, long chain fatty acids (LCFA) and glycerol, w...

Growth of sulfate reducing bacteria on the methanogenic inhibitor BES

The metabolism of methanogenic archaea is inhibited by 2-bromoethanesulfonate (BES). Methane production is blocked because BES is an analog of methyl-coenzyme M and competes with this key molecule in the last step of methanogenesis. For this reason, BES is commonly used in several studies to avoid growth of acetoclastic and hydrogenotrophic methanogens [1]. Despite its effectiveness as methanogenic inhibitor, B...

Proteomics of Syntrophomonas zehnderi and Methanobacterium formicicum growing o...

Background: Conversion of long-chain fatty acids (LCFA) in anaerobic digesters relies on syntrophic relationship between acetogenic bacteria and methanogenic archaea. Conversion of unsaturated- and saturated-LCFA has been previously shown by a coculture of Syntrophomonas zehnderi and Methanobacterium formicium. Degradation of unsaturated-LCFA is rare among Syntrophomonas species; the best studied fatty acid oxi...

Novel anaerobe obtained from a hexadecane-degrading consortium

Background: Aliphatic hydrocarbons (AHC) are abundant in crude oil and fuels, and are frequent contaminants of water, soil and sediments. There is potential for AHC bioremediation using sulfate as electron acceptor, due to its abundance in marine environments and natural presence in soils and groundwater. Objectives: In this work sulfate-reducing anaerobic microorganisms involved in AHC biodegradation were stud...

Prospecting fat-degrading anaerobes for biotechnology

Metaproteomics of anaerobic microbial communities degrading long-chain fatty acids

The anaerobic conversion of long-chain fatty acids (LCFA), and specifically the difference between the degradation of unsaturated- and saturated-LCFA, is not fully understood. In this work, syntrophic degradation of stearate (C18:0) and oleate (C18:1) was studied. A comparative metaproteomics approach, in which proteins were analyzed by LC-MS/MS, was combined with 16S rRNA gene pyrosequencing. Saturated- and un...