11 documents found, page 1 of 2
Purification, biochemical characterization, and amino acid sequence of a novel ...
Carneiro, Rômulo Farias; Torres, Renato Cézar Farias; Chaves, Renata Pinheiro; Vasconcelos, Mayron Alves de; Sousa, Bruno Lopes deA new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HClactivated Sepharose™ media. Hemagglutination caused by ADEL was inhibited by several galactosides, mainly galacturonic acid (Ka = 6.05 × 106 M−1 ). The primary structure of ADEL consists of 217 residues, including 11 halfcystines involved in five intrachain and one interchain disulfide bond, resulting in a molecular ...
Morphology, ultrastructure and immunocytochemistry of Hypnea cervicornis and Hy...
Miguel, Thaiz Batista Azevedo Rangel; Schmidt, Eder C.; Bouzon, Zenilda Laurita; Nascimento, Fernando E.P.; Cunha, Maura da; Fernandes, Saulo PiredaBased on their morphological and physiological features, red algae comprise a complex and variable group of multiple genera, including Hypnea. In particular, the genus Hypnea J.V. Lamouroux (Cystocloniaceae, Rhodophyta) consists of approximately 54 species, including Hypnea cervicornis and H. musciformis. Lectins were described for both species; however, the localization of these proteins is still unclear. Ther...
Purification, partial characterization and immobilization of a mannose-specific...
Pinto Júnior, Vanir Reis; Santiago, Mayara Queiroz de; Osterne, Vinícius José da Silva; Correia, Jorge Luis AlmeidaLectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-Dmannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,...
Purification and primary structure determination of a galactose-specific lectin...
Silva, Helton Colares; Nagano, C. S.; Souza, Luis A.G.; Nascimento, K. S.; Isídro, Renato; Delatorre, Plínio; Rocha, Bruno Anderson Matias daVatairea guianensis seeds, a typical plant from the Brazilian Amazon region that belongs to the Dalbergieae tribe, possess a lectin that was isolated by precipitation with solid ammonium sulfate followed by guar gum affinity chromatography. This lectin was named VGL. The V. guianensis lectin strongly agglutinated rabbit erythrocytes and was inhibited by d-galactose and d-galactose-derived sugars, especially N-a...
Structural basis for both pro- and anti-inflammatory response induced by mannos...
Rocha, Bruno Anderson Matias da; Delatorre, Plínio; Oliveira, Taianá Maia; Benevides, R. G.; Pires, Alana de Freitas; Sousa, Albertina A.S.Legume lectins, despite high sequence homology, express diverse biological activities that vary in potency and efficacy. In studies reported here, the mannose-specific lectin from Cymbosema roseum (CRLI), which binds N-glycoproteins, shows both pro-inflammatory effects when administered by local injection and anti-inflammatory effects when by systemic injection. Protein sequencing was obtained by Tandem Mass Sp...
Estudo preliminar sobre o sistema RNA-RNase em hepatopâncreas de lagosta jovem ...
Sampaio, Alexandre Holanda0 presente trabalho objetivou determinar as condigoes timas do sistema RNA-RNase, bem como as caracterlsticas basicas da enzima RNase em -hepatopancreas e sua rela gao com os estagios de muda de lagosta jovem Panulirus laevicauda (Latreille). 0 hepatopancreas foi retirado das lagostas vivas, estocadas em aquarios no Laboraterio de Ciencias do Mar, capturadas nas praias do Meireles e Titanzinho (Fortaleza - Cear...
In vitro evaluation of antioxidant activity of methanolic extracts obtained fro...
Lima, Rebeca Larangeira; Pires-Cavalcante, Kelma Maria dos S.; Alencar, Daniel Barroso; Viana, Francisco Analdo; Sampaio, Alexandre HolandaThe aim of the present research was to evaluate the antioxidant activity of six species of marine algae (Caulerpa cupressoides, Ulva fasciata, Amansia multifida, Cryptonemia crenulata, Dictyota dichotoma and Sargassum vulgare) collected in 2011 at Paracuru Beach, Ceará State, Brazil. Methanol extracts were prepared from oven-dried algae and used to measure total phenolic content (TPC) using the Folin- Ciocalteu...
Purification of a PHA-like chitin-binding protein from Acacia farnesiana seeds ...
Santi-Gadelha, Tatiane; Rocha, Bruno Anderson Matias; Oliveira, Cecília Carvalho; Aragão, Karoline Sabóia; Marinho, EEmmanuel SilvaA lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) followed by chromatofocusing. AFAL agglutinated rabbit erythrocytes and did not agglutinate human ABO...
Caracterização do sistema enzimático de hepatopâncreas de lagosta do gênero Pan...
Sampaio, Silvana Saker; Vieira, Gustavo Hitzschky Fernandes; Sampaio, Alexandre Holanda; Theophilo, Grace Nazareth DiogoSAMPAIO, Silvana Saker; VIEIRA, Gustavo Hitzschky Fernandes; SAMPAIO, Alexandre Holanda; THEOPHILO, Grace Nazareth Diogo. Caracterização do sistema enzimático de hepatopâncreas de lagosta do gênero Panulirus white.. Arquivos de Ciências do Mar, Fortaleza , v.28, 1989/1990, p.27-37; As lagostas Panulirus arguas (Latreille) e Panulirus laevicauda (Latreille) estão incluídas entre os principais recursos pesqueiros...
Caracterização preliminar do sistema enzimático de fígado de cangulo Balistes v...
Theophilo, Grace Nazareth Diogo; Vieira, Gustavo Hitzschky Fernandes; Sampaio, Silvana Saker; Sampaio, Alexandre HolandaTHEOPHILO, Grace Nazareth Diogo; VIEIRA, Gustavo Hitzschky Fernandes; SAMPAIO, Silvana Saker; SAMPAIO, Alexandre Holanda. Caracterização preliminar do sistema enzimático de fígado de cangulo Balistes vetula Linnaeus.. Arquivos de Ciências do Mar, Fortaleza, v.28, 1989/1990, p.9-16; O cangulo (Balistes vetula Linnaeus), peixe demersal encontrado em fundos coralinos e rochosos, participa com grande relevância na ...