133 documents found, page 1 of 14
Development of cytisus flower extracts with antioxidant and anti-inflammatory p...
Alvaredo-López-Vizcaíno, Adela; Costa-Barbosa, Augusto; Sampaio, Paula; Río, Pablo; Botelho, C. M.; Ferreira-Santos, PedroPlant flowers are recognized as a rich source of bioactive phenolic compounds. In this study, for the first time, the recovery of antioxidant phenolic compounds from Cytisus striatus flowers (CF) was optimized using microwave-assisted extraction (MAE). The variables (% of ethanol, temperature, and time) were studied using a response surface methodology (RSM). Extraction efficiency was assessed by total phenol c...
Characterizing the phenolic composition and exploring the antioxidant, anti-can...
Botelho, C. M.; Sousa, Diana F.; Quero, Javier; Costa-Barbosa, Augusto; Teixeira-Guedes, Catarina; Sampaio, Paula; Rodríguez-Yoldi, María JesúsNatural products and their derivatives have played important roles in various fields, including nutrition, medicine and cosmetics. In this study, different formulated plant infusions, namely Purifying Body (PB), Boost Training (BT), Calm Night I Spirit (CN), and Boost Energy (BE) were analyzed regarding their phenolic composition and biological properties such as antioxidant, anti-inflammatory, and its influenc...
Microwave-assisted extraction of raspberry pomace phenolic compounds, and their...
Jaouhari, Yassine; Bordiga, Matteo; Travaglia, Fabiano; Coisson, Jean-Daniel; Barbosa, Augusto Alexandre Costa; Sampaio, Paula; Botelho, C. M.Raspberry pomace (RBP) is rich in phenolic compounds. This study aims to optimize the extraction of phenolics from RBP and assess their bioaccessibility and bioactivity. The extraction process revealed that ethanol 50% was the most effective solvent. Microwave-assisted hydroethanolic extraction (MAE) significantly outperformed conventional methods, with optimal conditions of 200°C for 10min yielding the highest...
On the microstructural anisotropy and mechanical properties of bacterial nanoce...
Costa, Lígia; Carvalho, Alexandre F.; Fernandes, António J. S.; Campos, Teresa; Dourado, Nuno; Rodrigues, Ana Cristina; Sampaio, PaulaThe microstructural organization of bacterial nanocellulose is still not completely understood. Although its morphological and mechanical anisotropy is commonly recognized, it remains unclear whether the forces stabilizing the cellulose fiber network are comparable in different planes. In this study, we characterized the microstructure of statically produced BNC according to its horizontal and vertical planes. ...
Cellulose‑based hydrogel on quantum dots with molecularly imprinted polymers fo...
Piloto, Ana Margarida L.; Ribeiro, David S.M.; Rodrigues, S. Sofia M.; Santos, João L.M.; Sampaio, Paula; Sales, Maria Goreti FerreiraMolecularly imprinted polymers (MIPs), were successfully assembled around quantum dots (QDs), for the detection of the carbohydrate antigen CA19-9, a biomarker associated with pancreatic cancer (PC). The imprinted materials MIP@QDs were incorporated within cellulose hydrogels and designated as imprinted hydrogels (iHGs). The concept is to use MIPs which function as the biorecognition elements, conjugated to QDs...
Bacterial cellulose in vitro uptake by macrophages, epithelial cells, and a tri...
Carvalho, Ricardo Emanuel Silva; Rodrigues, Patrícia M.; Martins, Daniela Sofia Rodrigues; Rodrigues, Ana Cristina Costa; Sampaio, PaulaBacterial cellulose (BC) has a long-standing human consumption history in different geographies without any report of adverse effects. Despite its unique textural and functional properties, the use of BC in food products in Europe is still restricted due to concerns over its nanosize. Here, we evaluated the potential uptake of celluloses (from plant and microbial sources, processed using different blenders) by ...
Critical issues on the surface functionalization of plasmonic Au-Ag/TiO2 thin f...
Costa, Diogo; Pereira-Silva, Patrícia; Sousa, Paulo Jorge Teixeira; Pinto, Vânia Cristina Gonçalves; Borges, Joel; Vaz, Filipe; Minas, GraçaThis work reports on the surface functionalization of a nanomaterial supporting localized surface plasmon resonances (LSPRs) with (synthetic) thiolated oligonucleotide-based biorecognition elements, envisaging the development of selective LSPR-based DNA biosensors. The LSPR thin-film transducers are composed of noble metal nanoparticles (NPs) embedded in a TiO2 dielectric matrix, produced cost-effectively and s...
Candida albicans chitinase 3 with potential as a vaccine antigen: production, p...
Barbosa, Augusto Alexandre Costa; Ferreira, Diogo; Pacheco, Maria Inês; Casal, Margarida; Duarte, Henrique Oliveira; Gomes, CatarinaChitinases are widely studied enzymes that have already found widespread application. Their continued development and valorisation will be driven by the identification of new and improved variants and/or novel applications bringing benefits to industry and society. We previously identified a novel application for chitinases wherein the Candida albicans cell wall surface chitinase 3 (Cht3) was shown to have pote...
Combining fluconazole with benzo[a]phenoxazine derivatives as a promising strat...
Pacheco, Maria Inês Costa; Guimarães, Bárbara; Pereira-Silva, Patrícia; Costa-Barbosa, Augusto; Gonçalves, M. Sameiro T.; Sousa, Maria JoãoThe rise in non-albicans Candida species, exhibiting unpredictable antifungal resistance, complicates treatment and contributes to the growing threat of invasive, life-threatening infections. This study evaluates the antifungal activity of four benzo[a]phenoxazine derivatives (C34, C35, A42, and A44) against 14 Candida strains following EUCAST standards. Fluconazole interactions are analysed through fractional ...
Effects of Zn-ZnO Core-Shell Nanoparticles on Antimicrobial Mechanisms and Immu...
Fialho, Luísa; Costa-Barbosa, Augusto; Sampaio, Paula; Carvalho, SandraThe deposition of zinc-zinc oxide nanoparticles (Zn-ZnO NPs) onto porous Ta2O5 surfaces enriched with calcium phosphate by DC magnetron sputtering was investigated to improve the surface antimicrobial activity without triggering an inflammatory response. Different sizes and amounts of Zn NPs obtained by two optimized different depositions and an additional thin carbon (C) layer deposited over the NPs were explo...