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Revealing the diversity of the green Eulalia (Annelida, Phyllodocidae) species ...

Teixeira, Marcos André Machado Lima; Vieira, Pedro Emanuel Ferreira Reis; Fenwick, David; Langeneck, Joachim; Pleijel, Fredrik; Sampieri, Bruno R.

The green phyllodocids Eulalia clavigera and E. viridis are a known European pseudo-cryptic complex, but questions about its distribution and evidence of additional lineages in previous studies call for an investigation of the real diversity within the complex. We analyze DNA sequences (mtCOI-5P, ITS, and 28S rRNA) of different populations of E. clavigera from intertidal and subtidal marine waters along the Nor...


Reappraisal of the hyperdiverse Platynereis dumerilii (Annelida: Nereididae) sp...

Teixeira, Marcos André Machado Lima; Langeneck, Joachim; Vieira, Pedro Emanuel Ferreira Reis; Hernández, José Carlos; Sampieri, Bruno R.

Morphologically similar species are often overlooked but molecular techniques have been effective in signalling potential hidden diversity, boosting the documentation of unique evolutionary lineages and ecological diversity. Platynereis dumerilii and Platynereis massiliensis are part of a recognised species complex, where differences in the reproductive biology have mainly been highlighted to date. Analyses of ...


The curious and intricate case of the European Hediste diversicolor (Annelida, ...

Teixeira, Marcos André Machado Lima; Bakken, Torkild; Vieira, Pedro Emanuel Ferreira Reis; Langeneck, Joachim; Sampieri, Bruno R.; Kasapidis, Panagiotis

Past molecular studies using mtDNA sequences and alloenzymes signalled the existence of at least two cryptic species within the Hediste diversicolor morphotype, in European coasts. However, to this day, no new species descriptions have been made. In this study, we identified five completely sorted lineages using a multi-locus approach, including the mitochondrial DNA cytochrome oxidase I gene (COI-5P) and the n...


Molecular diversity within the genus Laeonereis (Annelida, Nereididae) along th...

Sampieri, Bruno R.; Vieira, Pedro E.; Teixeira, Marcos A. L.; Seixas, Victor C.; Pagliosa, Paulo R.; Amaral, Antonia Cecilia Z.; Costa, Filipe O.

The polychaete genus Laeonereis (Annelida, Nereididae) occurs over a broad geographic range and extends nearly across the entire Atlantic coast of America, from the USA to Uruguay. Despite the research efforts to clarify its diversity and systematics, mostly by morphological and ecological evidence, there is still uncertainty, mainly concerning the species Laeonereis culveri, which constitutes an old and notori...


Molecular and morphometric analyses identify new lineages within a large Eumida...

Teixeira, Marcos André Machado Lima; Vieira, Pedro Emanuel Ferreira Reis; Pleijel, Fredrik; Sampieri, Bruno R.; Ravara, Ascensão; Costa, Filipe O.

We report on two new lineages of the Eumida sanguinea complex from Great Britain and describe one of them as a new species using a multilocus approach, including the mitochondrial DNA COI-5P and the nuclear markers ITS (ITS1, 5.8S rRNA and ITS2) and 28S rRNA. The molecular analysis placed Eumida mackiei sp. nov. in a monophyletic clade with 19.1% (COI), 10.1% (ITS) and 1.7% (28S) mean distance to its nearest ne...


How oogenesis analysis combined with dna barcode can help to elucidate taxonomi...

Sampieri, Bruno R.; Steiner, Tatiana M.; Baroni, Priscila C.; da Silva, Camila Fernanda; Teixeira, Marcos André Machado Lima

Polychaetes are common in coastal and estuarine environments worldwide and constitute one of the most complex groups of marine invertebrates. The morpho-physiology of the female reproductive system (FRS) can be understood by using histological tools to describe reproductive cycle and gametogenesis paths and, among other purposes, aiming to identify and differentiate polychaete species. However, this histology-b...


Molecular and morphometric analyses identify new lineages within a large Eumida...

Teixeira, Marcos A. L.; Vieira, Pedro E.; Pleijel, Fredrik; Sampieri, Bruno R.; Ravara, Ascensão; Costa, Filipe O.; Nygren, Arne

We report on two new lineages of the Eumida sanguinea complex from Great Britain and describe one of them as a new species using a multilocus approach, including the mitochondrial DNA COI-5P and the nuclear markers ITS (ITS1, 5.8S rRNA and ITS2) and 28S rRNA. The molecular analysis placed Eumida mackiei sp. nov. in a monophyletic clade with 19.1% (COI), 10.1% (ITS) and 1.7% (28S) mean distance to its nearest ne...


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