3 documents found, page 1 of 1
Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a...
Fonseca, Fátima; Arthur, Christopher J.; Bromley, Elizabeth H. C.; Samyn, Bart; Moerman, Pablo; Saavedra, Maria José; Correia, António; Spencer, JamesThe subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a secon...
Protein differential expression induced by endocrine disrupting compounds in a ...
Lemos, Marco F. L.; Esteves, Ana Cristina; Samyn, Bart; Timperman, Isaak; van Beeumen, Jozef; Correia, António; van Gestel, Cornelis A. M.Endocrine disrupting compounds (EDCs) have been studied due to their impact on human health and increasing awareness of their impact on wildlife species. Studies concerning the organ-specific molecular effects of EDC in invertebrates are important to understand the mechanisms of action of this class of toxicants but are scarce in the literature. We have used a dose/response approach to unravel the protein expre...
Identification and proteolytic processing of procardosin A
Ramalho-Santos, Miguel; Veríssimo, Paula; Cortes, Luísa; Samyn, Bart; Beeumen, Jozef Van; Pires, Euclides; Faro, CarlosPlant aspartic proteinases contain a plant-specific insert (PSI) of about 100 amino acids of unknown function with no similarity with the other aspartic proteinases but with significant similarity with saposins, animal sphingolipid activator proteins. PSI has remained elusive at the protein level, suggesting that it may be removed during processing. To understand the molecular relevance of PSI, the proteolytic ...