6 documents found, page 1 of 1
Aptasensor for the detection of Moraxella catarrhalis adhesin UspA2
Sande, Maria; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Melo, Luís Daniel Rodrigues; Saragliadis, Athanasios; Linke, DirkInnovative point-of-care (PoC) diagnostic platforms are desirable to surpass the deficiencies of conventional laboratory diagnostic methods for bacterial infections and to tackle the growing antimicrobial resistance crisis. In this study, a workflow was implemented, comprising the identification of new aptamers with high affinity for the ubiquitous surface protein A2 (UspA2) of the bacterial pathogen Moraxella ...
Collagen-coated magnetic nanoparticles to capture pathogens
Sande, Maria; Roque, Lúcia; Marques, Márcia; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Braga, Adelaide Correia; Ramada, DiogoBackground The conventional method of diagnosing bacterial infections is based on microbial culture, however this method can take several hours or even days and lacks sensitivity. In the past few decades, the use of immunological and molecular techniques such as ELISA, PCR and mass spectrometry are used to diagnose infections. While these methods can be highly sensitive and reliable, they often require sample p...
New solutions to capture and enrich bacteria from complex samples
Sande, Maria; Çaykara, Tugçe; Silva, Carla Joana; Rodrigues, L. R.Current solutions to diagnose bacterial infections though reliable are often time-consuming, laborious and need a specific laboratory setting. There is an unmet need for bedside accurate diagnosis of infectious diseases with a short turnaround time. Moreover, low-cost diagnostics will greatly benefit regions with poor resources. Immunoassays and molecular techniques have been used to develop highly sensitive di...
Exploring the potential of polyethylene terephthalate in the design of antibact...
Caykara, Tugce; Sande, Maria; Azoia, Nuno; Rodrigues, L. R.; Silva, Carla JoanaPolyethylene terephthalate (PET) is one of the most used polymeric materials in the health care sector mainly due to its advantages that include biocompatibility, high uniformity, mechanical strength and resistance against chemicals and/or abrasion. However, avoiding bacterial contamination on PET is still an unsolved challenge and two main strategies are being explored to overcome this drawback: the anti-adhes...
Collagen-coated magnetic nanoparticles to capture pathogens from biological sam...
Sande, Maria; Roque, Lúcia; Marques, Márcia; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Braga, Adelaide; Ramada, David; Silva, C.Conventional methods of diagnosing bacterial infections such as microbial culture and molecular techniques, while highly sensitive, rely on expensive equipment and highly skilled operators. There is a need for affordable and portable diagnostic systems which are simple to operate while preserving reliability. Pre-enrichment of bacteria present in a sample coupled with subsequent bacterial identification steps c...
Design of materials to capture and enrich bacterial samples
Sande, Maria; Caykara, Tugce; Silva, Carla; Rodrigues, L. R.; Azoia, NunoBackground: Diseases arising from pathogenic infections cause immense loss of life and illnesses globally besides having severe negative social and economic impact. Current methods of diagnosing such infections rely mostly on culture-based assays which are complex, time consuming and expensive. Other related problems include low detection limits of diagnostic methods, thus requiring methods to concentrate and e...