7 documents found, page 1 of 1

Design of new hydrolyzed collagen-modified magnetic nanoparticles to capture pa...

Enrichment and diagnosis tools for pathogens currently available are time consuming, thus the development of fast and highly sensitive alternatives is desirable. In this study, a novel approach was described that enables selective capture of bacteria expressing hydrolyzed collagen-binding adhesins with hydrolyzed collagen-coated magnetic nanoparticles (MNPs). This platform could be useful to shorten the time ne...

Design of multifunctional materials to capture pathogens from biological samples

Diagnosis of bacterial infections is primarily accomplished by culture and laboratory analysis, immunological methods and nucleic-acid amplification techniques. However, these methods have limitations related to affordability, accessibility and time to inform a result. Moreover, the emergence of antibiotic resistant bacteria comprises a global threat to health, strengthening the relevance of early detection of ...

Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA o...

New point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on ...

Electrochemical aptasensor for the detection of the key virulence factor YadA o...

New point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on ...

Novel biorecognition elements against pathogens in the design of state-of-the-a...

Infectious agents, especially bacteria and viruses, account for a vast number of hospitalisations and mortality worldwide. Providing effective and timely diagnostics for the multiplicity of infectious diseases is challenging. Conventional diagnostic solutions, although technologically advanced, are highly complex and often inaccessible in resource-limited settings. An alternative strategy involves convenient ra...

DNA aptamers towards Yersinia enterocolitica and Moraxella catarrhalis adhesins...

Selection and validation of DNA aptamers against Yersinia Enterocolitica and Mo...