14 documents found, page 1 of 2
Design, synthesis and antiparasitic evaluation of click phospholipids
Magoulas, GE; Afroudakis, P; Georgikopoulou, K; Roussaki, M; Borsari, C; Fotopoulou, T; Santarém, N; Barrias, E; Tejera Nevado, P; Hachenberg, JA library of seventeen novel ether phospholipid analogues, containing 5-membered heterocyclic rings (1,2,3-triazolyl, isoxazolyl, 1,3,4-oxadiazolyl and 1,2,4-oxadiazolyl) in the lipid portion were designed and synthesized aiming to identify optimised miltefosine analogues. The compounds were evaluated for their in vitro antiparasitic activity against Leishmania infantum and Leishmania donovani intracellular ama...
Visceral dissemination of mucocutaneous leishmaniasis in a kidney transplant re...
Marques, N; Bustorff, M; Cordeiro-da-Silva, A; Pinto, AI; Santarém, N; Ferreira, F; Nunes, A; Cerqueira, A; Rocha, A; Ferreira, I; Tavares, I; Santos, JIntracellular protozoan of the genus Leishmania, endemic in the Mediterranean basin, are the cause of cutaneous (CL), mucocutaneous (MCL), and visceral leishmaniasis (VL). A 75-year-old woman was admitted nine years after a second kidney transplant (KT), due to persistent pancytopenia and fever. She presented edema and erythema of the nose in the last two years and an exophytic nodular lesion located on the lef...
Challenges in the serological evaluation of dogs clinically suspect for canine ...
Santarém, N; Sousa, S; Amorim, CMPG; Carvalho, NL; Carvalho, HL; Felgueiras, Ó; Brito, M; Cordeiro-da-Silva, ACanine leishmaniasis is a major veterinary issue and also a public health challenge due to its zoonotic potential. In this context, serological evaluation is essential for Canine leishmaniasis management. Several serological alternatives, such as rapid diagnostic tests, enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT), are well established. In fact, the capacity of distinct ...
The diverse piscidin repertoire of the european sea bass (Dicentrarchus labrax)...
Barroso, C; Carvalho, P; Carvalho, C; Santarém, N; Gonçalves, JFM; Rodrigues, P; Neves, JVFish rely on their innate immune responses to cope with the challenging aquatic environment, with antimicrobial peptides (AMPs) being one of the first line of defenses. Piscidins are a group of fish specific AMPs isolated in several species. However, in the European sea bass (Dicentrarchus labrax), the piscidin family remains poorly understood. We identified six different piscidins in sea bass, performed an in-...
Author Correction: Challenges in the serological evaluation of dogs clinically ...
Santarém, N; Sousa, S; Amorim, CMPG; Carvalho, NL; Carvalho, HL; Felgueiras, Ó; Brito, M; Cordeiro-da-Silva, AIn Figure 1C, D, and E the positive and negative controls are missing. The correct Figure 1 appears below.
More than just exosomes: distinct Leishmania infantum extracellular products po...
Perez-Cabezas, B; Santarém, N; Cecílio, P; Silva, C; Silvestre, R; A M Catita, J; Cordeiro-da-Silva, AThe use of secretion pathways for effector molecule delivery by microorganisms is a trademark of pathogenesis. Leishmania extracellular vesicles (EVs) were shown to have significant immunomodulatory potential. Still, they will act in conjunction with other released parasite-derived products that might modify the EVs effects. Notwithstanding, the immunomodulatory properties of these non-vesicular components and ...
Murine infection with bioluminescent Leishmania infantum axenic amastigotes app...
Costa, DM; Cecílio, P; Santarém, N; Cordeiro-da-Silva, A; Tavares, JLeishmaniasis is an important vector-borne neglected tropical disease caused by Leishmania parasites. Current anti-Leishmania chemotherapy is unsatisfactory, justifying the continued search for alternative treatment options. Herein, we demonstrate that luciferase-expressing Leishmania infantum axenic amastigotes, unlike promastigotes, are highly infectious to BALB/c mice and thus generate a robust bioluminescen...
The Use of Specific Serological Biomarkers to Detect CaniLeish Vaccination in Dogs
Lima, C; Santarém, N; Nieto, J; Moreno, J; Carrillo, E; Bartholomeu, D; Bueno, L; Fujiwara, R; Amorim, CMPG; Cordeiro-da-Silva, ACanine leishmaniosis (CanL) prevention in the Mediterranean basin is considered essential to stop human zoonotic visceral leishmaniasis. In this context, vaccination of dogs is expected to have a significant impact in disease control. CaniLeish® (Virbac Animal Health) is one of a few CanL vaccines that are at this moment licensed in Europe. This vaccine contains purified excreted-secreted proteins of Leishmania...
The use of Escherichia coli total antigens as a complementary approach to addre...
Lima, C; Mesquita, J; Brancal, H; Vahlenkamp, T; Teixeira, AR; Cardoso, L; Amorim, C; Santarém, N; Cordeiro-da-Silva, ACanine leishmaniosis (CanL) is a major veterinary concern and a public health issue. Serological data are essential for disease management. Several antigens used in serological assays have specificity related problems preventing relevant seropositivity values establishment. Herein we report significant seropositivity level disparity in a study cohort with 384 dogs from eight countries, for antigens traditionall...
Leishmania infantum exoproducts inhibit human invariant NKT cell expansion and ...
Belo, R; Santarém, N; Pereira, C; Pérez-Cabezas, B; Macedo, F; Leite-de-Moraes, M; Cordeiro-da-Silva, ALeishmania infantum is one of the major parasite species associated with visceral leishmaniasis, a severe form of the disease that can become lethal if untreated. This obligate intracellular parasite has developed diverse strategies to escape the host immune response, such as exoproducts (Exo) carrying a wide range of molecules, including parasite virulence factors, which are potentially implicated in early sta...