5 documents found, page 1 of 1
SAR study of 4,8-disubstituted Pyrimido[5,4-d]pyrimidines exhibiting antitrypan...
Lopes, André; Teixeira, Sofia; Santarém, Nuno; Greco, Alessandro; Pagliaro, Angela; Keminer, Oliver; Gul, Sheraz; Cordeiro-Da-Silva, AnabelaA set of new derivatives of 4,8-disubstituted pyrimido[5,4-d]pyrimidines were efficiently synthesized and in vitro evaluated against Trypanosoma brucei and Leishmania infantum promastigotes and intramacrophage amastigotes. The in vitro cytotoxicity was determined using the THP-1 cell line, and early in vitro ADME-Tox was carried out using in vitro assays for cytotoxicity (A549 and HEK293 cell lines) and CYP3A4 ...
Synthesis and biological evaluation of Amphotericin B formulations based on org...
Ferraz, Ricardo; Santarém, Nuno; Santos, Andreia F. M.; Jacinto, Manuel L.; Cordeiro-da-Silva, Anabela; Prudêncio, Cristina; Noronha, João PauloNowadays, organic salts and ionic liquids (OSILs) containing active pharmaceutical ingredients (APIs) are being explored as drug delivery systems in modern therapies (OSILs-API). In that sense, this work is focused on the development of novel OSILs-API based on amphotericin B through an innovative procedure and the evaluation of the respective biological activity against Leishmania infantum. Several ammonium, m...
Serological and Molecular Survey of Leishmania infantum in a Population of Iber...
Lima, Clara M.; Santarém, Nuno; Neves, Nuno Costa; Sarmento, Pedro; Carrapato, Carlos; de Sousa, Rita; Cardoso, Luís; Cordeiro-da-Silva, AnabelaLeishmania infantum, the sand fly-transmitted protozoan parasite responsible for leishmaniasis in humans, dogs, and cats, is endemic in the Iberian Peninsula. However, the impact of L. infantum infection on the conservation of the endangered Iberian lynx (Lynx pardinus) is unknown. Herein, we describe for the first time the occurrence of L. infantum infection among a population of reintroduced and wild-born L. ...
More than just exosomes: distinct Leishmania infantum extracellular products po...
Pérez-Cabezas, Begoña; Santarém, Nuno; Cecílio, Pedro; Silva, Cátia; Silvestre, Ricardo Jorge Leal; Catita, José A. M.; Cordeiro da Silva, AnabelaThe use of secretion pathways for effector molecule delivery by microorganisms is a trademark of pathogenesis. Leishmania extracellular vesicles (EVs) were shown to have significant immunomodulatory potential. Still, they will act in conjunction with other released parasite-derived products that might modify the EVs effects. Notwithstanding, the immunomodulatory properties of these non-vesicular components and ...
Immune response by nasal delivery of hepatitis B surface antigen and codelivery...
Borges, Olga; Cordeiro-da-Silva, Anabela; Tavares, Joana; Santarém, Nuno; Sousa, Adriano de; Borchard, Gerrit; Junginger, Hans E.Alginate coated chitosan nanoparticles were previously developed with the aim of protecting the antigen, adsorbed on the surface of those chitosan nanoparticles, from enzymatic degradation at mucosal surfaces. In this work, this new delivery system was loaded with the recombinant hepatitis B surface antigen (HBsAg) and applied to mice by the intranasal route. Adjuvant effect of the delivery system was studied b...