4 documents found, page 1 of 1
Hydrochar as protein support: preservation of biomolecule properties with non-c...
Castro, Manuela Oliveira; Santiago, Mayara Queiroz de; Nascimento, Kyria Santiago do; Cavada, Benildo Sousa; Miguel, Emilio de CastroIn this work, the ConBr lectin was non-covalently immobilized onto hydrochar (HC). This carbonaceous material was produced by the hydrothermal carbonization of glucose and then put to interact with the lectin, aiming to immobilize the biomolecule via electrostatic interactions. Samples obtained after the interaction were characterized by CHNS elemental analysis, scanning electron microscopy and Fourier transfor...
Purification, partial characterization and immobilization of a mannose-specific...
Pinto Júnior, Vanir Reis; Santiago, Mayara Queiroz de; Osterne, Vinícius José da Silva; Correia, Jorge Luis AlmeidaLectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-Dmannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,...
Análise estrutural da lectina de Dioclea lasiocarpa: uma proteína indutora de a...
Santiago, Mayara Queiroz deLectins are multidomain proteins with the ability to recognize carbohydrates in a specific way, in addition to interacting with molecules from other classes, such as hydrophobic compounds or secondary metabolites. Structural characterization of these molecules is crucial to understanding their function and activity in organisms and systems. An area with high potential of application for lectins is cancerology s...
Caracterização de bioconjugados de pontos quânticos de ZnCdTe com a lectina de ...
Santiago, Mayara Queiroz deIn recent years, researchers have focused their effort to the development of techniques with an interest in diagnostic strategies. To understand the onset and progression of various diseases before the action of any drug is necessary to evaluate the interactions of biological molecules in various cellular processes, this can be done by the use of biomarking strategies. Quantum dots (QDs) are fluorescent inorgan...