2 documents found, page 1 of 1
A novel and efficient and low-cost methodology for purification of Macrotyloma ...
Santana, Marcos Aurélio de; Santos, Alexandre Martins Costa; Oliveira, Marcelo Eurípedes; Oliveira, Jamil Silvano de; Babá, Élio HideoThe N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions...
Purification of a membrane-bound trypsin-like enzyme from the gut of the velvet...
Reis, Denise Torres Cruz; Mares-Guia, Thiago Rennó dos; Oliveira, Jamil Silvano de; Santos, Alexandre Martins Costa; Santoro, Marcelo MatosDisruption of protein digestion in insects by specific endoprotease inhibitors is being regarded as an alternative to conventional insecticides for pest control. To optimize the effectiveness of this strategy, the understanding of the endoprotease diversity of the target insect is crucial. In this sense, a membrane-bound trypsin-like enzyme from the gut of Anticarsia gemmatalis fifth-instar larvae was purified....