156 documents found, page 1 of 16

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Preservation methods in isolates of Sporothrix characterized by polyphasic appr...

de Souza Rabello, Vanessa Brito; Corrêa-Moreira, Danielly; Santos, Cledir; Abreu Pinto, Tatiana Casto; Procopio-Azevedo, Anna Carolina; Boechat, Jéssica

Sporotrichosis is a subcutaneous mycosis with worldwide distribution and caused by eight pathogenic species of the Sporothrix genus. Different ex situ preservation methods are used around the world to maintain the survival, morphophysiological and genetic traits of fungal strains isolated from patients with sporotrichosis for long terms. The main aim of the present study was to evaluate the survival, phenotypic...


Aflatoxins and ochratoxin A contamination during Merkén Pepper Powder productio...

Costa, Jéssica; Santos, Carla Isabel Arcanjo; Soares, Célia Maria Gonçalves; Lima, Nelson; Santos, Cledir

Berry fruits of Capsicum annuum L. cv. "Cacho de Cabra are used for the manufacture of a traditional pepper powder known as Merkén, which is a Chilean spicy widely consumed at national level in Chile and also exported to different countries. Merkén contamination with Aflatoxins (AFs) and Ochratoxin A (OTA) is a main concerning for local producers and national and worldwide consumers. In the present study, AFs a...


Capsicum spp. and the antifungal potential of capsaicinoids as safeguards for a...

Sepúlveda, M.; Cayún, Y.; Gallardo, V.; Costa, J.; Santos, Carla Isabel Arcanjo; Cornejo, P.; Lima, Nelson; Santos, Cledir

In recent years, cases of pathogenic fungi in agricultural crops and in animals, including humans, are growing (Paziani et al., 2020; Costa et al., 2022). Many of these pathogenic fungi are resistant to the commonly used antifungals. This scenario has created a need for new effective antifungals, particularly those based on eco-friendly approaches, such as plant secondary metabolites (e.g. capsaicinoids from Ca...


Characterisation of different Chilean Capsicum spp. varieties and the antifunga...

Sepúlveda, M.; Cayún, Y.; Gallardo, V.; Barría, E.; Nahuelcura, J.; Costa, J.; Dias, Nicolina Marques; Ruíz, A.; Cornejo, P.; Lima, Nelson

The increase in fungal resistance to synthetic antifungals used in agrifood production has brought the need to develop new technologies based on an eco-friendly approach. The main aim of this work was to evaluate the antifungal potential of Chilean Capsicum spp. extracts against plant pathogens and mycotoxigenic fungi found in agrifood production. Five different varieties of Chilean Capsicum spp. were obtained ...


Filamentous fungi isolated and identified from Antarctic soil (Fildes Bay, Anta...

Gallardo, V.; Sepúlveda, M.; Cayún, Y.; Barría, E.; Mella, M.; Costa, Jéssica; Dias, N.; Lima, Nelson; Cornejo, P.; Santos, Cledir

Purpose: Antarctica is a unique place with extreme environmental conditions, including low temperature, high solar radiation, low nutrient availability and strong winds. This different environment represents a gateway to studies on the taxonomy, ecology, and biotechnology of organisms under extreme conditions. Fungi are ubiquitous and diverse organisms in Antarctica and have been described as growing in differe...


Cotton textile with antimicrobial activity and enhanced durability produced by ...

Cisternas Novoa, C.; Tortella, G.; Seabra, A. B.; Diez, M.; Lima, Nelson; Santos, Cledir; Rubilar, O.

In this study, L-cysteine-capped silver nanoparticles (Cys-AgNPs) were successfully linked in a cotton textile, being attached in a covalent way to the cotton fibers via esterification with the hydroxyl groups from the cellulose. The AgNPs were strongly adhered to the fiber surface through coordination bonds with the thiol groups from the L-cys. In addition, they were compared with biogenic silver nanoparticles...


Antifungal potential of capsaicinoids and capsinoids from the capsicum genus fo...

Costa, Jéssica; Sepúlveda, Marcela; Gallardo, Víctor; Cayún, Yasna; Santander, Christian; Ruíz, Antonieta; Reyes, Marjorie; Santos, Carla Isabel Arcanjo

Opportunistic pathogenic fungi arise in agricultural crops as well as in surrounding human daily life. The recent increase in antifungal-resistant strains has created the need for new effective antifungals, particularly those based on plant secondary metabolites, such as capsaicinoids and capsinoids produced by <i>Capsicum</i> species. The use of such natural compounds is well-aligned with the One Health approa...


Occurrence of aflatoxins and Ochratoxin A during merkén pepper powder productio...

Costa, Jéssica; Santos, Carla Isabel Arcanjo; Soares, Célia Maria Gonçalves; Rodriguez, R.; Lima, Nelson; Santos, Cledir

Berry fruits of Capsicum annuum L. cv. “Cacho de Cabra” are used for the manufacture of a traditional pepper powder known as Merkén. In the present study, aflatoxins (AFs) and ochratoxin A (OTA) contamination in berry fruits of C. annuum was determined at harvest, drying, and smoking stages of Merkén production, in cumin and coriander seeds used as Merkén ingredients, and in the final packaged Merkén produced b...


An overview on possible links between aflatoxin B1 exposure and gallbladder cancer

Costa, Jéssica; Lima, Nelson; Santos, Cledir

Gallbladder cancer (GBC) is one of the most common sites for biliary tract cancers. It has a worldwide distribution being endemic in South America and Southern Asia. These high GBC rates have previously been linked to the determinants of health such as nutrition, genetics, lifestyle, and environment. Exposure to aflatoxin B1 (AFB1), a human carcinogen, is suggested to be involved with GBC development. This work...


MALDI-TOF MS: foundations and a practical approach to the clinically relevant f...

Gómez-Velásquez, Juan C.; Mojica-Figueroa, Iván L.; Santos, Cledir; Lima, Nelson; Mesa-Arango, Ana C.

In this review, the applicability of matrix-assisted laser ionisation/desorption time-of-flight mass spectrometry (MALDI-TOF) to the identification of clinical relevant filamentous fungi is presented. In addition, the need to implement and validate in-house databases, to fill the gaps in commercial databases, and the importance of permanently updating spectra databases are topics addressed.


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