Pseudomonas syringae pv. actinidiae is a pandemic agent of bacterial canker in kiwifruit. The virulence of bacteria can be mediated by horizontal gene transfer in a co-evolutionary process with other microorganisms, enriching their genetic repertoire and enhancing adaptation strategies and fitness. Temperate bacteriophages can integrate into bacterial genomes, increasing genetic diversity and potentially improv...
Pseudomonas syringae pv. actinidiae (Psa) is the causative agent of bacterial canker in kiwifruit (Actinidia spp.). Psa biovar 3 is the most prevalent and virulent, causing frequent and severe outbreaks worldwide. While current treatments have low efficacy, bacteriophages emerge as possible environmentally safe alternative biocontrol agents. In this study, bacteriophage Brt\_Psa3 was isolated from the soil of a...
The Mediterranean basin is the leading worldwide region for olive production. Extreme weather is increasingly frequent in this region, and how these climate extremes will affect olive products and quality remains unknown. We aim to study the effects of the water deficit stress, which occurred in the summer of 2017, on olive fruit and oil quality from a 30-year-old orchard. Fruits from olive trees standing on (i...
The influence of the colour of photo-selective nets on Actinidia deliciosa yield, fruit quality and progression of the bacterial kiwifruit canker (Pseudomonas syringae pv. actinidiae, Psa) need to be characterised due to increasing use of these nets, mainly to protect from hail and storms. From May 2019 onwards, pearl (Pn), yellow (Yn) and grey (Gn) nets were installed permanently in a ‘Hayward’ kiwifruit orcha...
Industrial applications of titanium dioxide nanoparticles (TiO2 NPs) are wide, and their use in nano-fertilizing technology has been proposed in the last few years. Bioactivity evaluation of different TiO2 NPs formulations is therefore crucial, not only to select the most appropriate formulation but also to validate potential agro-applications. In the current work, we compared the bioactivity of the two most us...
Cork oak (Quercus suber) is a species native to Mediterranean areas and its adaptation to the increasingly prevalent abiotic stresses, such as soil salinization, remain unknown. In sequence with recent studies on salt stress response in the leaf, it is fundamental to uncover the plasticity of roots directly exposed to high salinity to better understand how Q. suber copes with salt stress. In the present study w...
Bacterial canker of kiwifruit (Actinidia spp.) caused by Pseudomonas syringae pv. actinidiae (Psa), causes significant yield and financial losses. The use of copper-based products and antibiotics are the current techniques for Psa control. These compounds are phytotoxic and also promote copper and antibiotic resistance. The isolation and characterization of (bacterio)phages for the control of Psa was the focus ...
Background Fire blight is a destructive disease of pome trees, caused by Erwinia amylovora, leading to high losses of chain-of-values fruits. Major outbreaks were registered between 2010 and 2017 in Portugal, and the first molecular epidemiological characterization of those isolates disclosed a clonal population with different levels of virulence and susceptibility to antimicrobial peptides. Methods This work a...
Extreme climate events are increasingly frequent, and the 2017 summer was particularly critical in the Mediterranean region. Olive is one of the most important species of this region, and these climatic events represent a threat to this culture. However, it remains unclear how olive trees adjust the antioxidant enzymatic system and modulate the metabolite profile under field stress con-ditions. Leaves from two ...
Lead (Pb) soil contamination remains a major ecological challenge. Zygophyllum fabago is a candidate for the Pb phytostabilisation of mining tailings; nevertheless, the cytogenotoxic effects of low doses of Pb on this species are still unknown. Therefore, Z. fabago seeds collected from non-mining (NM) and mining (M) areas were exposed to 0, 5 and 20 M Pb for four weeks, after which seedling growth, Pb cytogenot...