Emulsifying properties of oil in water emulsions using quail egg white protein (Coturnix coturnix japonica) as an emulsifying agent were investigated using the conductivity technique. Changes in emulsion conductivity were recorded during and after homogenization. The results were interpreted in terms of properties related to the emulsifying activity and emulsion stability. The effect of salt concentrations (NaC...
Conjugates of protein (α-lactalbumin, β-lactoglobulin, and lysozyme) with polysaccharides (guar, locust, pectin, and carboxymethilcellulose) were prepared via Maillard reaction by the dry-heating method. The conjugates were characterizated by using the browning index, extent of reaction, grafting degree, sodium dodecyl sulfate – polyacrilamide gel electrophoresis, fluorescence, and circular dichroism. The emuls...
We investigated the extraction behavior of Cu(II) in the aqueous two-phase system (ATPS) formed by (L35 + MgSO4 + H2O) or (L35 + (NH4)2SO4 + H2O) in the presence of the extracting agent 1-(2-pyridylazo)-2-naphthol (PAN). At pH = 3 and a PAN concentration of 0.285 mmol kg−1, both ATPS lead to the effective separation of Cu(II) from other metallic ions (Zn(II), Co(II), Ni(II) and Fe(III)). High separation factors...
The study of protein interactions has generated great interest in the food industry. Therefore, research on new supramolecular structures shows promise. Supramolecular structures of the whey proteins α-lactalbumin and glycomacropeptide were produced under varying heat treatments (25 to 75 °C) and acidic conditions (pH 3.5to 6.5). Isothermal titration calorimetry experiments showed protein interactions and demon...
Phospholipases comprise a group of enzymes with applications in the thermal stability and emulsifying capacity of food formulations. In this work aqueous two-phase systems (ATPS) were used to extract phospholipase A2 (PLA2) from raw porcine pancreas and snake venom at 25 °C. PLA2 was partitioned into ATPS composed of inorganic salts, water and polyethylene glycol. We determined the partition coefficient, recove...
Protein–polysaccharide conjugates often display improved techno-functional properties when compared to their individual involved biomolecules. α-Lactalbumin:acacia gum (α-la:AG) conjugates were prepared via Maillard reaction by the dry-heating method. Conjugate formation was confirmed using results of absorbance, o-phthalaldehyde test, sodium dodecyl sulfate–polyacrilamide gel electrophoresis (SDS–PAGE) and siz...
The separation of Cd and Ni from Ni–Cd batteries using an aqueous two-phase system (ATPS) composed of copolymer L35, Li2SO4 and water is investigated. The extraction behavior of these metals from the bottom phase (BP) to the upper phase (UP) of the ATPS is affected by the amount of added extractant (potassium iodide), tie-line length (TLL), mass ratio between the phases of the ATPS, leaching and dilution factor...
The partitioning of the whey protein lactoferrin, which is an iron transporter glycoprotein, in an aqueous two-phase system composed of poly(ethylene glycol) (PEG) and sodium citrate was evaluated. Equilibrium data at 25 °C were determined for each system studied using PEG with a molar mass of 1000 and 4000 g·mol1 at pH values of 5.5, 6.5, and 7.5. An increase in the molar mass of the polymer promoted the expan...
Conselho Nacional de Desenvolvimento Científico e Tecnológico; Climate changes caused by increased atmospheric concentration of CO2 have stimulated a better understanding of the global carbon cycle. Soils represent one of the largest compartments of C on Earth, with higher C content than plant biomass and atmosphere. Therefore , understanding the interaction of one of the major compounds in soil organic matter ...