8 documents found, page 1 of 1
Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion...
Soares, Maria; Pinto, Maria M.; Nobre, Rui J.; Almeida, Luís Pereira de; Rasteiro, Maria da Graça; Santos, Teresa Almeida; Santos, João RamalhoFollicular fluid (FF) is the microenvironment where a growing oocyte develops. Intrafollicular communication ensures oocyte competence and is carried out through paracrine signaling, the exchange of molecules via gap junctions, and the trafficking of extracellular vesicles (EVs). The study of FF-derived EVs is important for both translational and fundamental research in the female reproductive field. This study...
Comics in Science and Health Communication: Insights From Mutual Collaboration ...
Tavares, Rui; Alemany-Pagès, Mireia; Araújo, Sara; Cohn, Neil; Santos, João Ramalho; Azul, Anabela MarisaComics have been used as a tool for learning, teaching, understanding, raising awareness and changing behaviours. Researchers are taking more advantage of this medium as comics in research has become a growing field. Notwithstanding, comics as research practice/method has received less attention, particularly the research framework involved in making comics. Here, we detail the research process through the draw...
Mitochondria as biological targets for stem cell and organismal senescence
Branco, Ana; Moniz, Inês; Santos, João RamalhoOrganismal aging is impacted by the deterioration of tissue turnover mechanisms due, in part, to the decline in stem cell function. This decline can be related to mitochondrial dysfunction and underlying energetic defects that, in concert, help drive biological aging. Thus, mitochondria have been described as a potential interventional target to hinder the loss of stem cell robustness, and subsequently, decreas...
In Vitro Evaluation of Biphasic Calcium Phosphate Scaffolds Derived from Cuttle...
Pereira, Patrícia; Neto, Ana S.; Rodrigues, Ana Sofia; Barros, Inês; Miranda, Catarina O.; Santos, João Ramalho; Almeida, Luís Pereira deThis study investigates the osteogenic differentiation of umbilical-cord-derived human mesenchymal stromal cells (hUC-MSCs) on biphasic calcium phosphate (BCP) scaffolds derived from cuttlefish bone doped with metal ions and coated with polymers. First, the in vitro cytocompatibility of the undoped and ion-doped (Sr2+, Mg2+ and/or Zn2+) BCP scaffolds was evaluated for 72 h using Live/Dead staining and viability...
New Insights on Sperm Function in Male Infertility of Unknown Origin: A Multimo...
Pacheco, Rita I.; Cristo, Maria I.; Anjo, Sandra I.; Silva, Andreia F.; Sousa, Maria Inês; Santos Tavares, Renata; Sousa, Ana PaulaThe global trend of rising (male) infertility is concerning, and the unidentifiable causes in half of the cases, the so-called unknown origin male infertility (UOMI), demands a better understanding and assessment of both external/internal factors and mechanisms potentially involved. In this work, it was our aim to obtain new insight on UOMI, specifically on idiopathic (ID) and Unexplained male infertility (UMI)...
Unhealthy lifestyles, environment, well-being and health capability in rural ne...
Azul, Anabela Marisa; Almendra, Ricardo; Quatorze, Marta; Loureiro, Adriana; Reis, Flávio; Tavares, Rui; Mota-Pinto, Anabela; Cunha, António; Rama, LuísBackground: Non-communicable diseases are a leading cause of health loss worldwide, in part due to unhealthy lifestyles. Metabolic-based diseases are rising with an unhealthy body-mass index (BMI) in rural areas as the main risk factor in adults, which may be amplified by wider determinants of health. Changes in rural environments reflect the need of better understanding the factors affecting the self-ability f...
Insights from qualitative research on NAFLD awareness with a cohort of T2DM pat...
Alemany-Pagès, Mireia; Moura-Ramos, Mariana; Araújo, Sara; Macedo, Maria Paula; Ribeiro, Rogério T.; do Ó, Dulce; Santos, João RamalhoIt is estimated that around 70% of Type 2 Diabetes Mellitus patients (T2DM) have Non-Alcoholic Fatty Liver Disease (NAFLD). Awareness and education are amongst the major shortcomings of the public health response to the increasing threat of NAFLD. Characterizing the specific NAFLD-related information needs of particular high-risk metabolic communities, for instance, T2DM patients, might aid in the development o...
Adição de piruvato de sódio e trolox ao diluidor utilizado para congelação de s...
Silva, Karen Mascaro Gonçalves da; Gamboa, Sandra Cristina de Oliveira Pinto; Rodrigues, Ana Sofia; Santos, João Ramalho; Guerra, Maria Madalena PessoaO objetivo deste trabalho foi avaliar o efeito do piruvato e trolox (forma solúvel da vitamina E) sobre a qualidade espermática pós-descongelamento. Assim, com o intuito de proteger as células espermáticas dos efeitos deletérios da criopreservação,foram considerados os seguintes tratamentos: T1 (Controle)= INRA82-HEPES sem antioxidantes; T2= INRA82-HEPES + 2mM de piruvato e T3= INRA82-HEPES + 120mM de trolox. A...