16 documents found, page 1 of 2
3D-MSCs A151 ODN-Loaded Exosomes Are Immunomodulatory And Reveal A Proteomic Ca...
P Camões, Sérgio; Bulut, Ozlem; Yazar, Volkan; Gaspar, Maria Manuela; Simões, Sandra; Ferreira, Rita; Vitorino, Rui; Santos, Jorge M.; Gursel, IhsanIntroduction Non-healing wounds remain a major burden due to the lack of effective treatments. Mesenchymal stem cell-derived exosomes (MSC-Exo) have emerged as therapeutic options given their pro-regenerative and immunomodulatory features. Still, little is known on the exact mechanisms mediated by MSC-Exo. Importantly, modulation of their efficacy through 3D-physiologic cultures together with loading strategies...
Gliding motility protein LIMP promotes optimal mosquito midgut traversal and in...
Egarter, Saskia; Santos, Jorge M.; Kehrer, Jessica; Sattler, Julia; Frischknecht, Friedrich; Mair, GunnarSubstrate-dependent gliding motility is key to malaria transmission. It mediates host cell traversal, invasion and infection by Plasmodium and related apicomplexan parasites. The 110 amino acid-long cell surface protein LIMP is essential for P. berghei sporozoites where it is required for the invasion of the mosquito’s salivary glands and the liver cells of the rodent host. Here we define an additional role for...
Functional genetic evaluation of DNA house-cleaning enzymes in the malaria para...
Kumar, Hirdesh; Kehrer, Jessica; Singer, Mirko; Reinig, Miriam; Santos, Jorge M.; Mair, Gunnar R.; Frischknecht, FriedrichBackground: Cellular metabolism generates reactive oxygen species. The oxidation and deamination of the deoxynucleoside triphosphate (dNTP) pool results in the formation of non-canonical, toxic dNTPs that can cause mutations, genome instability, and cell death. House-cleaning or sanitation enzymes that break down and detoxify non-canonical nucleotides play major protective roles in nucleotide metabolism and con...
Stacked Denoising Autoencoders and Transfer Learning for Immunogold Particles D...
Sousa, Ricardo; Santos, Jorge M.; Silva, Luís M.; Alexandre, Luís; Esteves, Tiago; Rocha, Sara; Monjardino, Paulo; Sá, Joaquim Marques deIn this paper we present a system for the detection of immunogold particles and a Transfer Learning (TL) framework for the recognition of these immunogold particles. Immunogold particles are part of a high-magnification method for the selective localization of biological molecules at the subcellular level only visible through Electron Microscopy. The number of immunogold particles in the cell walls allows the a...
Distribution-Based Categorization of Classifier Transfer Learning
Sousa, Ricardo Gamelas; Alexandre, Luís; Santos, Jorge M.; Silva, Luís M.; Sá, Joaquim Marques deTransfer Learning (TL) aims to transfer knowledge acquired in one problem, the source problem, onto another problem, the target problem, dispensing with the bottom-up construction of the target model. Due to its relevance, TL has gained significant interest in the Machine Learning community since it paves the way to devise intelligent learning models that can easily be tailored to many different applications. A...
High-Content Analysis of Breast Cancer Using Single-Cell Deep Transfer Learning
Kandaswamy, C.; Silva, L. M.; Alexandre, L. A.; Santos, Jorge M.High-content analysis has revolutionized cancer drug discovery by identifying substances that alter the phenotype of a cell, which prevents tumor growth and metastasis. The high-resolution biofluorescence images from assays allow precise quantitative measures enabling the distinction of small molecules of a host cell from a tumor. In this work, we are particularly interested in the application of deep neural ne...
Therapeutic angiogenesis induced by human umbilical cord tissue-derived mesench...
Pereira, Ana Rita S.; Mendes, Teresa F.; Ministro, Augusto; Teixeira, Mariana; Filipe, Mariana; Santos, Jorge M.; Bárcia, Rita N.; Goyri-O’Neill, J.Background: Mesenchymal stem cells derived from human umbilical cord tissue, termed UCX®, have the potential to promote a full range of events leading to tissue regeneration and homeostasis. The main goal of this work was to investigate UCX® action in experimentally induced hindlimb ischemia (HLI). Methods: UCX®, obtained by using a proprietary technology developed by ECBio (Amadora, Portugal), were delivered v...
Translational control of UIS4 protein of the host-parasite interface is mediate...
Silva, Patrícia A. G. C.; Guerreiro, Ana; Santos, Jorge M.; Braks, Joanna A. M.; Janse, Chris J.; Mair, GunnarUIS4 is a key protein component of the host-parasite interface in the liver stage of the rodent malaria parasite Plasmodium berghei and required for parasite survival after invasion. In the infectious sporozoite, UIS4 protein has variably been shown to be translated but also been reported to be translationally repressed. Here we show that uis4 mRNA translation is regulated by the P. berghei RNA binding protein ...
Translational repression of the cpw-wpc gene family in the malaria parasite Pla...
Rao, Pavitra N.; Santos, Jorge M.; Pain, Arnab; Templeton, Thomas J.; Mair, GunnarThe technical challenges of working with the sexual stages of the malaria parasite Plasmodium have hindered the characterization of sexual stage antigens in the quest for a successful malaria transmission-blocking vaccine. One such predicted and largely uncharacterized group of sexual stage candidate antigens is the CPW-WPC family of proteins. CPW-WPC proteins are named for a characteristic domain that contains...
Using neural networks and support vector regression to relate marchetti dilatom...
Cruz, Manuel; Santos, Jorge M.; Cruz, NunoIn the last two decades, small strain shear modulus became one of the most important geotechnical parameters to characterize soil stiffness. Finite element analysis have shown that in-situ stiffness of soils and rocks is much higher than what was previously thought and that stress-strain behaviour of these materials is non-linear in most cases with small strain levels, especially in the ground around retaining ...