6 documents found, page 1 of 1
Isolation and characterization of 25 microsatellite DNA loci for Anopheles albi...
Guimarães-Marques, Giselle Moura; Batista, Jacqueline da Silva; Guimarães, H. M.; Naice-Daou, M. V.; Lima, M. P.; Formiga, Kyara M.The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosit...
Mutations in the voltage-gated sodium channel gene of anophelines and their ass...
Silva, Ana Paula B.; Santos, Joselita Maria M.; J, Martins, AdemirConstant and extensive use of chemical insecticides has created a selection pressure and favored resistance development in many insect species worldwide. One of the most important pyrethroid resistance mechanisms is classified as target site insensitivity, due to conformational changes in the target site that impair a proper binding of the insecticide molecule. The voltage-gated sodium channel (NaV) is the targ...
Differentiation and genetic variability in natural populations of Anopheles (N....
Santos, Joselita Maria M.; Maia, Juracy de Freitas; Tadei, Wanderli PedroPopulations of Anopheles triannulatus from Macapá (AP), Aripuanã (MT), Ji-Paraná (RO), and Manaus-Janauari Lake (AM) were studied using 16 enzymatic loci. The results of the isozyme analysis showed that the population of Macapá presented higher polymorphism (56.3%). The lowest variability was observed in the population of Manaus (p = 25.0; Ho = 0.077 +/- 0.046). The results of Wright's F statistics showed unbal...
Isolation and characterization of polymorphic DNA microsatellite loci for Anoph...
Cruz-Hernández, Pedro; Batista, Jacqueline da Silva; Formiga, Kyara M.; Rafael, Míriam Silva; Tadei, Wanderli Pedro; Santos, Joselita Maria M.Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited...
Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchu...
Borges-Moroni, R.; Fraga, Elmary da Costa; Maia, Juracy de Freitas; Tadei, Wanderli Pedro; Santos, Joselita Maria M.Our group analyzed species of Anopheles of the subgenus Nyssorhynchus (An. darlingi, An. marajoara, An. oswaldoi, An. benarrochi, An. triannulatus and An. rangeli) and a single species of the subgenus Anopheles (An. mattogrossensis), originating in the Amazon, based on the mitochondrial DNA control region. The fragment had 381 pb and the nucleotide composition in A+T ranged from 85.8 to 89.5%. DNA polymorphism ...
Ecologic observations on anopheline vectors of malaria in the Brazilian amazon
Tadei, Wanderli Pedro; Thatcher, Bedsy Dutary; Santos, Joselita Maria M.; Scarpassa, Vera Margarete; Rodrigues, Iléa Brandão; Rafael, Míriam SilvaHuman intervention in the Brazilian Amazon region promotes contacts between humans and vectors that may favor the propagation of anopheline mosquitoes and the spread of malaria in the absence of planning and infrastructure to control this disease. Vector ecology studies were carried out to determine the risk areas. These data should help in designing appropriate malaria control measures. Data from 14 different ...