7 documents found, page 1 of 1
Magnetic-based strategies for regenerative medicine and tissue engineering
Santos, Lúcia F.; Silva, Ana S.; Mano, João F.The fabrication of biological substitutes to repair, replace, or enhance tissue- and organ-level functions is a long-sought goal of tissue engineering (TE). However, the clinical translation of TE is hindered by several challenges, including the lack of suitable mechanical, chemical, and biological properties in one biomaterial, and the inability to generate large, vascularized tissues with a complex structure ...
Biomimetic adhesive micropatterned hydrogel patches for drug release
Amaral, Katia R.; Silva, A. Sofia; Santos, Lúcia F.; Castanheira, Edgar J.; Mendes, Maria C.; Costa, Dora C. S.; Rodrigues, João M. M.; Marto, JoanaThe optimized physical adhesion between bees' leg hairs and pollen grains-whereby the latter's diameter aligns with the spacing between the hairs-has previously inspired the development of a biomimetic drug dressing. Combining this optimized process with the improved natural mussels' adhesion in wet environments in a dual biomimetic process, it is herein proposed the fabrication of a natural-derived micropatter...
Freestanding magnetic microtissues for tissue engineering applications
Santos, Lúcia F.; Patrício, Sónia G.; Silva, Ana Sofia; Mano, João F.A long-sought goal in tissue engineering (TE) is the development of tissues able to recapitulate the complex architecture of the native counterpart. Microtissues, by resembling the functional units of living structures, can be used to recreate tissues' architecture. Howbeit, microfabrication methodologies fail to reproduce cell-based tissues with uniform shape. At the macroscale, complex tissues are already pro...
Bioengineered hierarchical bonelike compartmentalized microconstructs using nan...
Bjørge, Isabel M.; Sousa, Bárbara M. de; Patrício, Sónia G.; Silva, Ana Sofia; Nogueira, Liebert P.; Santos, Lúcia F.; Vieira, Sandra I.Fabrication of vascularized large-scale constructs for regenerative medicine remains elusive since most strategies rely solely on cell self-organization or overly control cell positioning, failing to address nutrient diffusion limitations. We propose a modular and hierarchical tissue-engineering strategy to produce bonelike tissues carrying signals to promote prevascularization. In these 3D systems, disc-shaped...
Cell-based soft biomaterials
Silva, A. Sofia; Santos, Lúcia F.; Oliveira, Mariana B.; Mano, João F.Cell-based tissue engineering (TE) has been rapidly evolving to meet the demand of scaffold-free regenerative tissues. Commonly used scaffold-based strategies are often associated with delayed tissue formation, rejection and tissue failure. Scaffold-free TE is a bottom-up methodology that makes use of cell-based soft biomaterials such as cell sheets(CS), spheroids or multishaped microtissues and fiber-shaped mu...
Multi-layer pre-vascularized magnetic cell sheets for bone regeneration
Silva, Ana S.; Santos, Lúcia F.; Mendes, Maria C.; Mano, João F.The lack of effective strategies to produce vascularized 3D bone transplants in vitro, hampers the development of thick-constructed bone, limiting the translational of lab-based engineered system to clinical practices. Cell sheet (CS) engineering techniques provide an excellent microenvironment for vascularization since the technique can maintain the intact cell matrix, crucial for angiogenesis. In an attempt t...
Complex-shaped magnetic 3D cell-based structures for tissue engineering
Santos, Lúcia F.; Silva, A. Sofia; Mano, João F.The use of cells as building blocks for tissue engineering purposes has been a matter of research in the recent years. Still, the fabrication of complex-shaped 3D-like constructs using living-based materials is hampered through the difficulty in recapitulating the mechanical properties of the native tissues. In an attempt to develop robust tissue-like constructs, it is herein proposed the fabrication of complex...