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Insight into the efficiency of microalgae’ lipidic extracts as photosensitizers...

Mendonça, Inês; Silva, Daniela; Conde, Tiago; Maurício, Tatiana; Cardoso, Helena; Pereira, Hugo; Bartolomeu, Maria; Vieira, Cátia; Domingues, M. Rosário

Antibacterial resistance causes around 1.27 million deaths annually around the globe and has been recognized as a top 3 priority health threat. Antimicrobial photodynamic therapy (aPDT) is considered a promising alternative to conventional antibiotic treatments. Algal lipid extracts have shown antibacterial effects when used as photosensitizers (PSs) in aPDT. In this work we assessed the photodynamic efficiency...


Short-communication: study of fatty acid metabolites in microbial conjugated fa...

Fontes, Ana Luiza; Neves, Bruna; Conde, Tiago; Couto, Daniela; Pimentel, Lígia Leão; Rodríguez-Alcalá, Luis Miguel; Domingues, M. Rosário

Microbially enriched food in conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids is intensively studied nowadays. The conversion of linoleic (LA) and α-linolenic acids (α-LNA) into these compounds may involve different fatty acid (FA) intermediates. This research aimed to investigate potential FA byproducts in milk during microbial CLA/CLNA-enrichment using Bifidobacterium breve DSM 20091. Milk ferm...


Microbial conjugated linolenic acid-enriched fermented milk using lipase-hydrol...

Fontes, Ana Luiza; Pimentel, Lígia L.; Monteiro, Maria João P.; Domingues, M. Rosário; Rodríguez-Alcalá, Luis Miguel; Gomes, Ana Maria

The bioactive conjugated linolenic acid (CLNA) can be microbiologically produced by different probiotic strains when in the presence of α-linolenic acid (α-LNA). Food matrices are a good vector, such as has been previously demonstrated with fermented milk enriched with microbial CLNA by Bifidobacterium breve DSM 20091 from lipase-hydrolyzed flaxseed oil. The aim of the present work was to further assess the nut...


Insight into the efficiency of microalgae’ lipidic extracts as photosensitizers...

Mendonça, Inês; Silva, Daniela; Conde, Tiago; Maurício, Tatiana; Cardoso, Helena; Pereira, Hugo; Bartolomeu, Maria; Vieira, Cátia; Domingues, M. Rosário

Antibacterial resistance causes around 1.27 million deaths annually around the globe and has been recognized as a top 3 priority health threat. Antimicrobial photodynamic therapy (aPDT) is considered a promising alternative to conventional antibiotic treatments. Algal lipid extracts have shown antibacterial effects when used as photosensitizers (PSs) in aPDT. In this work we assessed the photodynamic efficiency...


Use of Chlorella vulgaris lipidic extracts in the development of healthier past...

Pereira, Tatiana; Barroso, Sónia; Teixeira, Paula; Domingues, M. Rosário; Maurício, Tatiana; Mendes, Susana; Pinto, Filipa R.; Freire, Catarina D.

Pastry products constitute a significant segment of the food market. However, the high amount of fat used in their production poses a challenge when competing for the attention of modern consumers, who are more conscious of the health problems associated with the consumption of high-fat products. With this in mind, the main objective of this study is the reduction of the total fat and saturated fat contents of ...


New solvent-free method for isolating ellagic acid from sulphite spent liquor

Evtyugin, Dmitry D.; Prates, António; Domingues, M. Rosário; Casal, Susana; Evtyugin, Dmitry V.

Ellagic acid (EA) is a plant polyphenol with wide potential in biomedical (e.g., anti-inflammatory, neuroprotective, and anti-melanogenic, among others) and technical applications such as copolymers, chelating reagents, ion-exchange resins and materials for electrochemical devices. Upon acid sulphite pulping of Eucalyptus wood, EA is partially dissolved in the spent liquor and adsorbed on the resulting pulp. In...


Optimization of Pavlova gyrans biomass production and the fatty acid profile us...

Maciel, Filipe; Couto, Daniela; Geada, Pedro; Pereira, Hugo; Teixeira, José; Domingues, M. Rosário; Silva, Joana; Vicente, António

This work aimed to optimize and characterize the biomass production of Pavlova gyrans through a sequential multivariate approach. Among seventeen cultivation parameters, light intensity, NaNO3, and CuSO45H(2)O were identified as the most significative factors (p < 0.10) for biomass productivity. Together with NaH2PO4H2O, these variables were further studied in order to maximize the biomass production of P. gyra...


Fatty acid profiles of cultured hippocampus hippocampus trunk muscles and poten...

Cabral, Ana Elisa; Rey, Felisa; Domingues, M. Rosário; Cabral, Miguel; Planas, Miquel; Palma, Jorge; Calado, Ricardo

Syngnathids (seahorses, pipefishes and seadragons) are an attractive resource for Traditional Chinese Medicine (TCM). Despite few scientific studies supporting seahorse nutritional benefits, they are believed to possess medicinal properties that enhance human health. The European short-snout seahorse Hippocampus hippocampus is classified as Data Deficient by the IUCN Red List of Threatened Species. Nevertheless...


Light modulates the lipidome of the photosynthetic sea slug Elysia timida

Rey, Felisa; Cartaxana, Paulo; Aveiro, Susana; Greenacre, Michael; Melo, Tânia; Domingues, Pedro; Domingues, M. Rosário; Cruz, Sónia

Long-term kleptoplasty, the capability to retain functional stolen chloroplasts (kleptoplasts) for several weeks to months, has been shown in a handful of Sacoglossa sea slugs. One of these sea slugs is Elysia timida, endemic to the Mediterranean, which retains functional chloroplasts of the macroalga Acetabularia acetabulum. To understand how light modulates the lipidome of E. timida, sea slug specimens were s...


Optimization of Pavlova gyrans biomass production and the fatty acid profile us...

Maciel, José Filipe Gonçalves; Couto, Daniela; Geada, Pedro Miguel Macedo; Pereira, Hugo; Teixeira, J. A.; Domingues, M. Rosário; Silva, Joana

This work aimed to optimize and characterize the biomass production of Pavlova gyrans through a sequential multivariate approach. Among seventeen cultivation parameters, light intensity, NaNO3, and CuSO4·5H2O were identified as the most significative factors (p < 0.10) for biomass productivity. Together with NaH2PO4·H2O, these variables were further studied in order to maximize the biomass production of P. gyra...


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